新诱变剂甲基丙烯酸环氧丙酯对质粒限制酶酶谱的改变  

New Mutagen, Glycidyl Methacrylate (GMA) Changed Restriction Enzyme Map of Plasmid

作　　者：左瑾[1] 高惠兰[2] 杨惠芳[2] 谢大英[2] 李忠生[2] 方福德[1] 

机构地区：[1]中国医学科学院基础医学研究所 [2]中国预防医学科学院劳动卫生与职业病研究所

出　　处：《中国医学科学院学报》1991年第2期144-147,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金

摘　　要：甲基丙烯酸环氧丙酯(glycidyl methacrylate,GMA)作为粘合剂的主要成分,在我国广泛应用于航空及其它工业,对环境造成一定污染.可归入环境诱变剂类物质.已知GMA不仅能与哺乳类基因组DNA发生共价结合,在一定浓度下还可诱导人和大鼠血淋巴细胞及雄性小鼠配子细胞的DNA修复合成,以及抑制S期DNA半保留复制.进一步的研究发现GMA能与质粒pBR322结合,并引起其损伤。Our present work showed that the mutant TcS ApR of plasmid pBR322 induced by GMA. changed restriction enzyme map in the single and double enzyme digestions. Results indicated that the mutant plasmid has lots of, mutated sites which are disappeared on some sites and appeared on others in resistance gene region, for example, sites of BgⅠ, EcoRⅠ. HindⅢ and HincⅡ, and occured a new recognition site for Hindi (252). The relat ionship between gene mutation and genotoxicity of GMA was discussed.

关 键 词：环氧丙酯 甲基丙烯酸 限制酶 酶谱 

分 类 号：Q319.33[生物学—遗传学]