氯过氧化物酶催化对氨基苯磺酸降解及其环境适应性研究  

Research on the catalytic degradation process of p-aminobenzenesulfonic acid by chloroperoxidase

作　　者：卢靖[1,2] 蒋育澄[1] 

机构地区：[1]陕西师范大学化学化工学院,陕西西安710119 [2]陕西科技大学材料科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报》2018年第1期28-33,共6页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金项目(21176150)

摘　　要：本实验研究氯过氧化物酶催化对氨基苯磺酸降解过程中,不同介质pH值,H_2O_2浓度,反应时间,酶浓度等对降解效率的影响.通过高效液相色谱-质谱联用分析降解产物的化学组成并推测降解路线.通过降解前后溶液中单细胞绿藻生长情况评价降解产物的环境毒性,进一步在模拟工业废水环境中进行催化降解应用实验.结果表明:氯过氧化酶催化对氨基苯磺酸降解的最佳条件为介质pH=4.0,H_2O_2浓度3mmol·L-1,氯过氧化酶用量4μmol·L-1,反应时间10min,此时底物的降解率达到96%.对氨基苯磺酸可通过两种途径分解成小分子产物,被氯过氧化物酶催化降解之后环境毒性很小.应用实验显示,工业废水中的金属离子对酶活性有抑制作用,最佳降解率为64%.In this study,the catalytic degradation process of p-aminobenzenesulfonic acid by chloroperoxidase was investigated.The effects of media pH,H_2O_2 concentration,reaction time,and chloroperoxidase dosage on the removal of p-aminobenzenesulfonic acid were discussed in details.The chemical composition of the products were determined by high performance liquid chromatography-mass spectrometry.And the possible degradation process was discussed as well.The environmental toxicity was evaluated by the growth of single cell green algae.Furthermore,the catalytic process was carried out in the industrial wastewater for application.The results showed chloroperoxidase could remove p-aminobenzenesulfonic acid effectively.The optimum processing conditions are as follows,the H_2O_2 concentration was 3 mmol·L-1,pH=4.0,reacting for 10 min,dosage of chloroperoxidase was 4μmol·L-1.The degradation rate of the p-aminobenzenesulfonic acid reached to 96%.It is supposed that p-aminobenzenesulfonic acid can be decomposed to little molecules through two different processes.The environmental toxicity of chloroperoxidase catalysis is very weak.The metal ions in industrial wastewater have inhibitory effect on the enzyme activity,and the optimum degradation rate is 64%.

关 键 词：氯过氧化物酶 对氨基苯磺酸 催化降解 

分 类 号：X131[环境科学与工程—环境科学]