蛹虫草固体培养产子实体条件的优化及其虫草素含量测定  

Optimization of Solid Culture for Fruiting Body Production of Cordyceps militaris and Determination of Its Cordycepin Content

作　　者：刁朝强 文庭池 廖勇 康冀川 周建云 谢红艳[3] 

机构地区：[1]贵州省烟草公司贵阳市公司,贵州贵阳550025 [2]贵州大学西南药用生物资源教育部工程研究中心,贵州贵阳550025 [3]山东省农业科学院农业资源与环境研究所农业部废弃物基质化利用重点实验室山东省农业面源污染防控重点实验室,山东济南250100

出　　处：《山地农业生物学报》2017年第6期86-89,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州省烟草公司贵阳市公司项目(No.2013-04);贵州省中药现代化科技产业研究开发专项项目(黔科合中药字[2012]5008号)

摘　　要：本研究采用大米米饭固体培养基培养出蛹虫草子实体,在菌株、培养基、接种方式三个因素中筛选出高产蛹虫草子实体的最优组合,并与工业液体发酵蛹虫草菌丝体作比较,用高效液相色谱法(High Performance Liquid Chromatography,HPLC)测定了子实体和采后培养基中的虫草素含量,结果表明,子实体中的虫草素含量(10.22 mg/g)是采后培养基中虫草素含量(2.04 mg/g)的5倍,是工业液体发酵蛹虫草菌丝体中虫草素含量(7.06 mg/g)的1.45倍。In this study,fruiting bodies of Cordyceps militaris were successfully cultured on rice solid medium.Different strains,culture medium and inoculation method were optimized in order to improve the yield of fruiting bodies of C.militaris.Moreover,the content of cordycepin in fruiting bodies and postharvest rice medium was analyzed using high performance liquid chromatography compared with that of industrial liquid fermentative mycelia.The result showed that,the content of cordycepin in fruiting bodies(10.22 mg/g) was 5 times higher than that in postharvest rice solid medium(2.04 mg/g) and 1.45 times higher than that in industrial liquid fermentative mycelia(7.06 mg/g).

关 键 词：蛹虫草 大米米饭培养基 高效液相色谱法 优化 虫草素 

分 类 号：Q939[生物学—微生物学]