Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test  

作　　者：Yilin Liu Chen Li Lei Huang Yun He Tingting Zhao Bo Han Xiaoqiang Jia 

机构地区：[1]Department of Biochemical Engineering School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China [2]Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, China [3]Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China

出　　处：《Chinese Journal of Chemical Engineering》2017年第12期1838-1846,共9页中国化学工程学报（英文版）

基　　金：Supported by the National Basic Research Program of China(“973”Program:2014CB745100);the National Natural Science Foundation of China(21576197);Tianjin Research Program of Application Foundation and Advanced Technology(14JCQNJC06700);the Major Research Plan of Tianjin(16YFXTSF00460);Tianjin Penglai 19-3 Oil Spill Accident Compensation Project(19-3BC2014-03)

摘　　要：Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were isolated from petroleum-contaminated soil and water in Tianjin Binhai New Area Oil field, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudornonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achrornobacter sp., Acinetobacter venetianus, Lysinibacillus rnacroides, Klebsiella oxytoca, Stenotrophornonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline en- vironments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were isolated from petroleum-contaminated soil and water in Tianjin Binhai New Area Oilfield, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudomonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achromobacter sp., Acinetobacter venetianus, Lysinibacillus macroides, Klebsiella oxytoca, Stenotrophomonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline environments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.

关 键 词：Crude oil-degrading strains Biosurfactant producer Environmental tolerance Pilot-scale bioremediation 

分 类 号：X172[环境科学与工程—环境科学]