癫痫幼鼠海马组织中大麻素受体2表达变化  

The change of Cannabinoid receptor 2 expression in childhood rat with epilepsy

作　　者：曹庆隽[1] 刘雪雁[1] 杨凤华[1] 孙晶晶[1] 于涛[1] 王华[1] 

机构地区：[1]中国医科大学附属盛京医院小儿神经内科,沈阳110004

出　　处：《中国小儿急救医学》2017年第12期906-911,共6页Chinese Pediatric Emergency Medicine

基　　金：辽宁省科学技术计划（2014225007）

摘　　要：目的探讨幼年大鼠癫痫持续状态后不同时间的大麻素受体2（CB2receptor，CB2R）的动态变化。方法健康雄性SD大鼠80只（18～21日龄），随机分配成2组：对照组和癫痫组，又依癫痫持续状态后不同时间分为2h、24h、14d、21d共4个时间点，应用免疫组织化学、PCR和Westernblot检测不同时间点大鼠海马组织CB2RmRNA及蛋白质表达情况。结果对照组大鼠随着日龄增加，大脑海马内的CB2R含量逐渐增多，到日龄35～42d时逐渐趋于平稳，癫痫组在癫痫持续状态后2h一14d海马区CB2R的含量较对照组多，癫痫持续状态后2h癫痫组的CB2RmRNA表达与对照组比较，差异有统计学意义（1．878比2．062，P〈0．05）；癫痫持续状态后24h及14d两组比较差异均有统计学意义（均P〈0．05）；癫痫持续状态后21d对照组的CB2RmRNA表达较癫痫组略多（6．018比5．938），差异无统计学意义（P〉0．05）。结论CB2R在癫痫持续状态下幼年癫痫大鼠海马区呈现高表达，随癫痫发作后时间的延长达到高峰，之后逐渐下降。Objective To investigate the changes of cannabinoid 2 receptor （CB2R） expression at different time after epileptic status in epileptic childhood rats. Methods Healthy male SD rats （ 18-21 days old） were randomly divided into two groups ： control group and epileptic model group. CB2R concentration in hippocampus of rats were tested at 2 h, 24 h, 14 d, 21 d after entering the status epilepticus （ status epilepsy, SE） by immunohistochemistry, PCR, Western blot. Results In control group, CB2R content of the hippo- campus brain gradually increased with age increasing. When the rats with the age of 35-42 days ,CB2R con- tent gradually got stabilized. After status epilepticus for 2 h-14 d, CB2R content of hippocampus in epileptic model group was more than that in the control group. At the point of 21 d,CB2R content of hippocampus in the control group was more than that in epileptic model group. CB2R mRNA of epileptic model group at 2 hours point was more than that of control group （2. 062 vs 1. 878 ,P 〈0. 05）. At 24 h and 14 d after SE,there were significant differences between two groups in CB2R mRNA respectively （ P 〈 0. 05, respectively）. At 21 d after SE,CB2R mRNA of control group was more than that of epileptic group （6.018 vs 5. 938） ,but there was no significant difference between two groups （ P 〉 0. 05 ）. Conclusion CB2R presents high expression in childhood rat hippocampus suffering from status epilepticus, reaches a peak following with prolonged seizures, then gradually decreased.

关 键 词：癫痫 大麻素受体2 癫痫持续状态 

分 类 号：R742.1[医药卫生—神经病学与精神病学]