神经营养因子3与酪氨酸激酶受体C在卒中后抑郁模型大鼠额前皮质的表达  

The expression of NT-3 and TrkC in prefrontal cortex of the post-stroke depression model rats

作　　者：李云 李姝 王玮 杨宁 李漾超 

机构地区：[1]大理大学第一附属医院神经内科,大理671000 [2]大理大学临床医学院,大理671000

出　　处：《中华行为医学与脑科学杂志》2017年第12期1064-1069,共6页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：云南省科技厅科研基金项目（2009ZC126M）

摘　　要：目的 探讨神经营养因子3（neurotrophic factor-3,NT-3）及其受体酪氨酸激酶受体C （tyrosine kinase receptors,TrkC）mRNA及蛋白在脑卒中后抑郁（post stroke depression,PSD）模型大鼠额前皮质的表达变化.方法 将实验大鼠进行行为学评分（Open-field法）,评分相近的大鼠被分为4组：正常组、抑郁组、卒中组及PSD组,每组13只.抑郁组：采用孤养法结合慢性不可预见的中等应激刺激（chronic unpredictable mild stress,CUMS）建立;卒中组：利用线栓法建立局灶性脑缺血大鼠模型;PSD组：动物建成脑缺血模型后孤养再加以CUMS方法建立PSD模型,并与正常对照组、卒中组及抑郁组作比较.于造模后第29天应用免疫组化及RT-PCR检测各组大鼠额前皮质NT-3及其高亲和力受体TrkC的免疫阳性细胞及mRNA的表达情况.结果 免疫组化实验结果发现,PSD组额前皮质NT-3免疫阳性细胞数[（10.11±2.89）个/视野]较正常对照组明显减少[（19.00±12.41）个/视野] （P〈0.05）,其余各组相比差异无统计学意义（P〉0.05）;PSD组大鼠额前皮质TrkC免疫阳性细胞数[（19.56±5.66）个/视野]较正常对照组[（25.11±3.95）个/视野]及脑卒中组[（29.67±7.38）个/视野]明显减少（P〈0.05）;抑郁组免疫阳性细胞数[（19.00±5.61）个/视野]也较正常对照组[（25.11±3.95）个/视野]及脑卒中组（29.67±7.38个/视野）明显减少（P〈0.05）;其余各组相比差异无统计学意义（P〉0.05）.RT-PCR检查结果显示各组大鼠额前皮质组织中均有 GAPDH、NT3和 TrkCmRNA的表达, PSD组大鼠额前皮质NT-3的表达明显低于正常对照组（P〈0.05）;其余各组比较差异无统计学意义（P〉0.05）;各组TrkCmRNA表达差异无统计学意义（P〉0.05）.结论 PSD大鼠额前皮质NT-3及其受体TrkC的表达减少可能与PSD发病有关.Objective To explore the expression of NT-3 and high-affinity receptors TrkC at mRNA and protein level in the prefrontal cortex of the post stroke depression（PSD）model rats.Methods Open-field method was used to evaluate the behavior.Focal cerebral ischemic rat models were made with thread em-bolization method.PSD rat models were established with comprehensive separately breeding and chronic un-predicted mild stress（CUMS）on this basis.Normal control group,depression group and stroke group were used to compare with PSD group.13 rats were used in each group.At 29th day after the CUMS RT-PCR was employed to detect gene expression of NT-3 and TrkC.The expression of NT-3 and TrkC in positive cells was detected by immunohistochemistry.Results The immunohistochemistry results showed that the number of NT-3 immunopositive cells in PSD group（10.11± 2.89）was lower than that of the normal group（19.00 ± 12.41）（P〈0.05）.Whereas there was no statistical difference in the other groups（P〉0.05）.The number of TrkC immunopositive cells in PSD group（19.56±5.66）was less than that of the normal group（25.11±3.95） and stroke group（29.67±7.38）.The number of TrkC immunopositive cells in depression group（19.00±5.61）also was lower than that of the normal group（25.11±3.95）and stroke group（29.67±7.38）.There was no sta-tistical difference among other groups（P〉0.05）.RT-PCR results showed that the GAPDH,NT-3 and TrkC mRNA in all of the groups could be detected in the prefrontal cortex of rats.The expression of NT-3 in the prefrontal cortex in the PSD group decreased significantly compared with that of normal control rats（P〈0.05）.There was no statistical difference in the other groups（P〉0.05）.The expression of TrkC in the pre-frontal cortex had no statistical difference in all of the groups（P〉0.05）.Conclusion The down-regulation of NT-3 and TrkC both at mRNA and protein levels in the prefrontal cortex maybe responsible for the pathogen-esis of PSD.

关 键 词：脑卒中后抑郁 额前皮质 神经营养因子3 酪氨酸激酶受体C 

分 类 号：R-332[医药卫生] R743.3