条件性恐惧记忆相关基因的生物信息学分析  

Bioinformatics analysis of fear conditioning memory related genes

作　　者：刘欢 顾小萍[1] 

机构地区：[1]南京医科大学鼓楼临床医学院麻醉科,南京210008

出　　处：《中华行为医学与脑科学杂志》2017年第12期1076-1080,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81371207）

摘　　要：目的 利用生物信息学方法对公共基因数据库微阵列表达谱进行分析,确定条件性恐惧记忆的关键基因.方法 从Gene Expression Omnibus（GEO）下载GSE57465微阵列数据,数据分为对照组和实验组（FC_0.5 h组和FC_2 h组）.在R软件中通过分位数法对原始数据进行标准化,然后通过芯片数据平台的注释包（illuminaMousev2.db）将探针名称转换为基因名.选用Limma方法筛选对照组和实验组之间的差异表达基因,差异表达基因纳入标准：Padj〈0.05,差异倍数〉1.5.之后对差异表达基因进行聚类分析,以及GO和KEGG富集分析.构建差异表达基因所翻译的蛋白质之间相互作用（protein-protein interactions,PPI）网络,并选取网络中包含枢纽蛋白的网络模块进行分析,确定网络中的相关枢纽蛋白.结果 初步筛选了47个差异表达基因,其中FC_0.5 h组含有36个差异表达基因;FC_2 h组含有11个差异表达基因.GO和KEGG分析提示差异表达基因主要富集于核糖体,参与核糖体内蛋白合成的信号通路,同时也发挥着正调控RNA聚合酶II启动子转录和翻译的生物学作用.PPI网络分析发现Egr2,Fos,Sgk1三个蛋白质的表达水平显著升高,并在网络中发挥枢纽蛋白的作用.结论 Egr2,Fos,Sgk1可能在条件性恐惧记忆的形成中起重要作用.Objective To identify key genes associated with fear conditioning memory by bioinfor-matics analysis of gene expression profiles from Gene Expression Omnibus（GEO）. Methods Downloaded from GEO,GSE57465 microarray data were divided into control group and experiment groups（FC_0.5 h group and FC_2h group）.The original data was normalized in the R software by the quantile method,and then converted the probe names to the gene names through the annotation package（illumina Mouse v2.db）.The differentially expressed genes（DEGs）between the control and experimental groups were screened by the Limma method,selected Padj〈0.05 and Fold Change〉1.5 as DEGs.Clustering analysis,as well as GO and KEGG enrichment analysis were performed in DEGs.At last,DEGs translated proteins interaction network were established,and the key genes in the network were identified.Results 47 DEGs were screened,among them,FC_0.5h group consisted of 36 DEGs and FC_2h group consisted of 11 DEGs.GO and KEGG enrich-ment analysis suggested that DEGs were mainly enriched in ribosomes,participating in the signal pathway of protein synthesis in ribosomes,and also playing a biological role in the transcription and translation of posi-tive regulatory RNA polymerase II promoter.PPI network analysis showed that Egr2,Fos and Sgk1 which sig-nificantly increased,may play as hub proteins in the network. Conclusion Egr2,Fos,Sgk1 may play an im-portant role in the formation of fear conditioning memory.

关 键 词：条件性恐惧记忆 生物信息学 基因分析 聚类分析 

分 类 号：B842.3[哲学宗教—基础心理学]