Topology: a unique dimension in protein engineering  

作　　者：Lianjie Xu Wen-Bin Zhang 

机构地区：[1]Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Center for Soft Matter Science and Engineering,College of Chemistry and Molecular Engineering, Peking University

出　　处：《Science China Chemistry》2018年第1期3-16,共14页中国科学（化学英文版）

基　　金：supported by the National High Technology Research and Development Program of China (2015AA020941);the National Natural Science Foundation of China (21474003, 91427304);"1000 Plan (Youth)"

摘　　要：Controlling protein topology has been a long standing challenge to go beyond their linear configuration defined by the translation mechanism of cellular machinery. In this mini-review, we focus on the topological diversity in proteins and review the major categories of protein topologies known to date, including branched/star proteins, circular proteins, lasso proteins, knotted proteins, and protein catenanes. The discovery of these topologically complex natural proteins and their synthetic pathways, the rational design and recombinant synthesis of artificial topological proteins and their biophysical studies, are summarized and discussed with regard to their general features and broad implications. The complexity of protein topology is recognized and the routes to diverse protein topologies are illustrated. We believe that topology engineering is an important way to modify protein properties without altemating their native sequences and shall bring in valuable dynamic features central to the creation of artificial protein machinery.Controlling protein topology has been a long standing challenge to go beyond their linear configuration defined by the translation mechanism of cellular machinery. In this mini-review, we focus on the topological diversity in proteins and review the major categories of protein topologies known to date, including branched/star proteins, circular proteins, lasso proteins, knotted proteins,and protein catenanes. The discovery of these topologically complex natural proteins and their synthetic pathways, the rational design and recombinant synthesis of artificial topological proteins and their biophysical studies, are summarized and discussed with regard to their general features and broad implications. The complexity of protein topology is recognized and the routes to diverse protein topologies are illustrated. We believe that topology engineering is an important way to modify protein properties without alternating their native sequences and shall bring in valuable dynamic features central to the creation of artificial protein machinery.

关 键 词：protein topology protein engineering CATENANE ROTAXANE KNOT lasso peptide 

分 类 号：N0[自然科学总论—科学技术哲学]