口腔扁平苔藓病损组织中白细胞介素35的表达及意义  

Expression and significance of interleukin-35 in lesion tissue of oral lichen planus patients

作　　者：王荣 刘芳 江南 蔡扬 

机构地区：[1]贵州医科大学附属口腔医院牙周黏膜科,贵阳550004

出　　处：《中华口腔医学杂志》2018年第1期13-19,共7页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81460104）

摘　　要：目的 通过检测口腔扁平苔藓（oral lichen planus,OLP）病损组织中白细胞介素（interleukin,IL）35两个亚基EB病毒诱导基因3（Epstein-Barr virus-induced gene 3,EBi3）及IL-12p35的表达情况,探讨IL-35在OLP病损形成及发展中的作用及意义.方法 选取2010年10月至2016年12月贵州医科大学附属口腔医院牙周黏膜科门诊41例OLP组织（OLP组,非糜烂型21例,糜烂型20例）,15例正常对照组织（健康对照组）,采用实时荧光定量PCR法（quantitatvie real-time PCR, qPCR）检测OLP局部病损组织中EBi3、IL-12p35 mRNA的表达情况,采用免疫组织化学方法检测OLP局部病损组织中蛋白EBi3、IL-12p35的分布与表达;分析IL-35与OLP临床病理特征的关系.结果OLP病损组织中EBi3、IL-12p35 mRNA的相对表达量[M（Q25,Q75）分别为3.38（1.63,11.25）、6.39（2.55, 14.30）]均显著高于健康对照组[分别为1.41（0.33,3.16）、2.47（1.10,5.14）]（Z=-2.806,P=0.005;Z=-2.276,P=0.023）.OLP病损组织中EBi3、IL-12p35蛋白的阳性表达率[分别为66%（27/41）、39% （16/41）]均显著高于健康对照组[0%（0/15）]（P〈0.05）;且EBi3与IL-12p35蛋白的相对表达量呈正相关（r=0.404,P=0.009）.EBi3蛋白的阳性表达率与基底细胞液化程度有关（χ2=9.172,P=0.010）;IL-12p35蛋白的阳性表达率在糜烂型OLP中的表达显著高于非糜烂型（χ2=7.220,P=0.007）.IL-35蛋白在OLP病损组织中的阳性表达率为34%（14/41）,显著高于健康对照组（χ2=6.829,P=0.009）,且在糜烂型OLP中的表达[50%（10/20）]显著高于非糜烂型[19%（4/21）]（χ2=4.364,P=0.037）.结论 OLP局部病损组织中IL-35表达上调,与OLP的临床分型有关,提示IL-35可能在OLP病损形成中起重要作用.Objective To investigate the expression of Epstein-Barr virus-induced gene 3 （EBi3） and interleukin-12p35 （IL-12p35） in two subunits of interleukin-35 （IL-35） in oral lichen planus （OLP） lesions,and to explore the role of IL-35 played in the formation and development of OLP lesions.Methods Totally 41 samples of OLP lesions and 15 samples of normal tissues were collected from patients of the Department of Periodontology and Oral Medicine, Affiliated Stomatological Hospital of Guizhou Medical University from October 2010 to December 2016. The expression levels of EBi3 mRNA and IL-12p35 mRNA in the samples were detected by quantitative real-time PCR （qPCR） and the distribution and expression of protein EBi3 and IL-12p35 were detected by immunohistochemistry.The potential relationship between IL-35 and clinicopathological features of OLP was analyzed.Results The expression[M（Q25,Q75）] of EBi3 [3.38 （1.63, 11.25）] and IL-12p35 mRNA [6.39 （2.55, 14.30）] in OLP lesion tissues were significantly higher than those in normal control group [1.41 （0.33, 3.16）, 2.47 （1.10, 5.14）] （Z=-2.806, P=0.005;Z=-2.276,P=0.023）,respectively.The positive expression rates of EBi3 and IL-12p35 were 66% （27/41）and 39%（16/41）,respectively,were significantly higher in OLP lesion tissues comparing with that in normal oral mucosa tissues [0%（0/15）] （P〈0.05）. The relative expressions of EBi3 and IL-12p35 were positively correlated （r=0.404, P=0.009）. A significant correlation was found between EBi3 protein over expression and the degeneration of base cells in OLP lesions（x2=9.172,P=0.010）.The positive expression rate of IL-12p35 protein in erosive type lesions was higher than that in non-erosive type lesions （x2=7.220, P=0.007）. The positive expression rate of IL-35 protein in OLP lesions [34% （14/41）] was higher than that in normal control group （x2=6.829, P=0.009）. The expression rate of IL-35 in erosive type lesions （10/20） was significantly higher than that

关 键 词：扁平苔藓 口腔 口腔黏膜 白细胞介素35 

分 类 号：R781.5[医药卫生—口腔医学]