HSYA与芍药苷联用对脑缺血再灌注损伤大鼠PI3K、Akt mRNA表达的影响  

HSYA Combined with Paeoniflorin Has Effect on Expressions of PI3K and Akt mRNA in Rats with Cerebral Ischemia Reperfusion Injuries

作　　者：谭燕萍 周福宜 梁慧超[2] 余梦黎 廖金明[4] 郑丽娴[4] 姚晖 秦莎莎 张继平 

机构地区：[1]佛山市禅城区朝阳医院,广东佛山528000 [2]佛山市第一人民医院脑科康复医院,广东佛山528000 [3]广东医科大学药学院,广东湛江524023 [4]南方医科大学中医药学院,广东广州510515 [5]南方医科大学附属佛山医院,广东佛山528000 [6]广东药科大学中药学院,广东广州510006

出　　处：《新中医》2018年第1期11-15,共5页New Chinese Medicine

基　　金：佛山市科技攻关项目(2015AB001491;2016AB002981)

摘　　要：目的:探讨羟基红花黄色素A(HSYA)与芍药苷联合用药对急性局灶性脑缺血再灌注损伤大鼠PI3K/Akt信号通路中PI3K、Akt mRNA表达的影响。方法:SPF级雄性SD大鼠60只,随机分为6组,每组10只,分别为模型组、假手术组、HSYA组、芍药苷组、银杏内酯组、合用组。复制脑缺血再灌注模型。脑缺血1 h再灌注6 h后尾静脉注射相应的药物7天,每天1次。HSYA组灌胃HSYA溶液5.0 mg/(kg·d);芍药苷组灌胃芍药苷溶液5.0 mg/(kg·d);合用组是HSYA与芍药苷联合用药,灌胃HSYA溶液和芍药苷溶液各5.0 mg/(kg·d);银杏内酯组灌胃银杏内酯注射液5.0 mg/(kg·d);模型组和假手术组灌胃等量生理盐水。末次给药2 h后采集相应标本保存,qRT-PCR法检测大鼠海马组织PI3K、Akt mRNA的表达。结果:与假手术组比较,模型组大鼠PI3K mRNA表达量显著减少,差异有统计学意义(P<0.01)。与模型组比较,HSYA组、芍药苷组、合用组、银杏内酯组大鼠海马组织中PI3K mRNA的表达显著降低,差异均有统计学意义(P<0.05)。与合用组比较,HSYA组、银杏内酯组大鼠PI3K mRNA的表达量较高,差异均有统计学意义(P<0.05)。结论:HSYA与芍药苷联合用药对脑缺血再灌注损伤模型大鼠的保护机制可能与显著调节PI3K/Akt信号通路中PI3K mRNA过表达,微调Akt mRNA过表达有关。Objective： To observe the effect of hydroxy safflower yellow A（HSYA） combined with paeoniflorin on expressions of PI3K and Akt mRNA in PI3K/Akt signal pathway of rats with acute focal cerebral ischemia reperfusion injuries.Methods： Divided 60 SPF male SD rats into 6 groups,namely the model group,sham operation group,HSYA group,paeoniflorin group,Ginkgolide group,and the combination group,10 rats in each group.Replicated the model of cerebral ischemia reperfusion.After one hour of cerebral ischemia and 6 hours of perfusion the rats were medicated related intravenous injection from posterior caudal vein for 7 days,once a day,continuously HSYA group was given 5.0 mg/（kg·d） of HSYA solution by gavage.Paeoniflorin group was given 5.0 mg/（kg·d） of paeoniflorin solution by gavage.The combination group received 5.0 mg/（kg·d） of HSYA combined with 5.0 mg/（kg·d） of paeoniflorin by gavage.Ginkgolide group was given 5.0 mg/（kg·d） of Ginkgolide injection by gavage.The model group and sham operation group were given the same amount of normal saline.Collected corresponding samples after 2 hours of the last time of administration,and then reserved them.Detected expressions of PI3K and Akt mRNA in hippocampus of rats by qRT-PCR（quantitative real-time polymerase chain reaction） method.Results：PI3K mRNA expression levels in rats in the model group were obviously reduced in comparison with those in the sham operation group,the difference being significant（P〈0.01）.PI3KmRNA expression levels in hippocampus of rats in HSYA group,paeoniflorin group,Ginkgolide group,and the combination group were evidently declined by comparing with those in the model group,differences being significant（P〈0.05）.PI3K mRNA expression levels of rats were higher in HSYA group and Ginkgolide group in comparison with those of the combination group,differences being significant（P〈0.05）.Conclusion：The protection mechanism of the combined medications of HSYA and paeoniflorin for rat model of cerebral isch

关 键 词：脑缺血再灌注损伤 羟基红花黄色素A(HSYA) 芍药苷 PI3K MRNA AKT MRNA 动物实验 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]