血清miR-663、miR-126和miR-370对冠心病患者支架内再狭窄的预测价值  

Predictive value of serum miR-663,miR-126 and miR-370 for in-stent restenosis in patients with coronary heart disease

作　　者：王芳[1] 李蓓[1] 刘成伟[1] 吴明祥[1] 苏晞[1] 

机构地区：[1]武汉亚洲心脏病医院心内科,湖北省武汉市430022

出　　处：《中国动脉硬化杂志》2017年第11期1138-1142,1168,共6页Chinese Journal of Arteriosclerosis

基　　金：武汉市卫计委课题(WX14C78)

摘　　要：目的研究血清微小RNA(miRNA)中的miR-663、miR-126和miR-370在冠心病患者支架植入术后的表达情况,分析以上3种miRNA对支架内再狭窄(ISR)的预测价值。方法研究纳入175例冠心病支架植入术后并随访复查冠状动脉造影的患者,根据造影结果分为ISR组(n=66)和无ISR组(n=109)。分析比较两组患者的临床资料。收集所有患者的血清,通过实时荧光定量PCR检测血清miR-663、miR-126和miR-370的表达情况。应用统计学方法分析以上3个miRNA预测ISR的价值。结果 175例支架植入术后冠心病患者中发生ISR的共66例。ISR组吸烟率更高(P<0.05);ISR组血糖、低密度脂蛋白胆固醇、高敏C反应蛋白(hs-CRP)水平均高于无ISR组(P<0.01)。ISR组血清miR-663、miR-126表达量低于无ISR组,而miR-370表达量高于无ISR组(均P<0.01)。Logistic回归分析表明,miR-663、miR-126表达水平与ISR发生相关,吸烟、hs-CRP是ISR的独立危险因素。受试者工作特征曲线结果表明,miR-663、miR-126和miR-370预测ISR的曲线下面积(AUC)分别为0.772、0.720和0.650;而miR-663联合miR-126预测ISR的AUC为0.909,优于单一miRNA的预测效能。结论冠心病支架植入术后ISR患者血清miR-663、miR-126表达下降,miR-370表达增加。miR-663、miR-126和miR-370可用于预测ISR的发生,miR-663联合miR-126可明显提高预测ISR的效能。Aim To investigate the serum expressions of miR-663,miR-126 and miR-370 of microRNA（miRNA） after stent implantation in patients with coronary heart disease,and to analyze the predictive value of the above 3 types of miRNA in in-stent restenosis（ISR）. Methods The study included 175 patients with coronary heart disease,who underwent coronary stent implantation and followed up coronary angiography,and the patients were divided into ISR group（n= 66） and non-ISR group（n= 109） according to the results of angiography. The clinical data were analyzed and compared in the two groups. The serum samples of all patients were collected and the expression levels of serum miR-663,miR-126 and miR-370 were detected by real-time quantitative PCR. The values of the above 3 types of miRNA predicting ISR were analyzed by statistical methods. Results A total of 66 cases of ISR occurred in 175 patients with coronary heart disease after stent implantation. Smoking rate was higher in ISR group（P〈0.05）. The levels of blood glucose,low density lipoprotein cholesterol and high-sensitivity C-reactive protein（hs-CRP） in ISR group were higher than those in nonISR group（P〈0.01）. The expressions of serum miR-663 and miR-126 in ISR group were lower than those in non-ISR group,but the expression of miR-370 was higher than that in non-ISR group（all P〈0.01）. Logistic regression analysis showed that the expression levels of miR-663 and miR-126 were related to ISR,and smoking and hs-CRP were independent risk factors of ISR. The results of receiver operating characteristic curve showed that the area under curve（AUC） of miR-663,miR-126 and miR-370 predicting ISR were 0.772,0.720 and 0.650,respectively,while the AUC of miR-663 combined with miR-126 predicting ISR was 0.909,which was superior to the prediction efficiency of single miRNA. Conclusion The expressions of miR-663 and miR-126 were decreased and the expression of miR-370 is increased in patients with ISR after coronary stent implantation. Serum miR-663,mi

关 键 词：冠心病 支架内再狭窄 微小RNA 

分 类 号：R0[医药卫生]