肥大细胞在金黄色葡萄球菌肠毒素B诱导小鼠特应性皮炎样炎症反应中的作用  

Role of mast cells in Staphylococcus aureus enterotoxin B- induced atopic dermatitis- like skin inflammation in mice

作　　者：王珊[1] 赵作涛[1] 王誉涵 涂平[1] 刘玲玲[1] 

机构地区：[1]北京大学第一医院皮肤科皮肤病分子诊断北京市重点实验室,100034

出　　处：《中华皮肤科杂志》2018年第1期26-30,共5页Chinese Journal of Dermatology

摘　　要：目的 探讨肥大细胞在金黄色葡萄球菌肠毒素B（SEB）诱导的BALB/c小鼠特应性皮炎样炎症反应中的作用。方法 24只BALB/c小鼠分为4组，分别使用卵清蛋白（OVA）、SEB、OVA ＋ SEB联合组及生理氯化钠溶液（对照组）建立经皮诱导的AD样模型。对模型小鼠AD样皮损进行临床观察和皮损严重程度评分，组织病理切片并行甲苯胺蓝及免疫组化染色观察肥大细胞的数目、分布及形态，计算肥大细胞脱颗粒百分比。结果 分别单独使用OVA、SEB及OVA ＋ SEB联合组经皮诱导模型小鼠AD样局部炎症反应临床表现、皮损严重程度评分及组织病理炎症细胞浸润程度，均较对照组严重，且OVA ＋ SEB组较OVA及SEB组更为严重（P〈0.05）。AD样皮损真皮的肥大细胞数目分别为10.625（3.675）/高倍视野、11.000（4.163）/高倍视野和13.875（8.813）/高倍视野，均较对照组的5.925（2.088）/高倍视野明显增多（P〈0.05）；SEB组和OVA ＋ SEB组的肥大细胞脱颗粒百分比达（71.083 ± 14.519）%和（58.767 ± 16.978）%，高于OVA组（24.050 ± 11.161）%和对照组（23.617 ± 8.132）%（P〈0.05）。二元变量相关分析显示，各组皮损肥大细胞总数与临床皮损严重程度评分呈线性相关（P〈0.05）。结论 SEB经皮刺激可诱导小鼠产生AD样皮损，并可加重OVA诱导的小鼠AD样皮损严重程度。肥大细胞增殖、活化脱颗粒释放类胰蛋白酶等参与炎症过程。Objective To investigate the role of mast cells in Staphylococcus aureus enterotoxin B （SEB）-induced atopic dermatitis（AD）-like skin inflammation in BALB/c mice. Methods A total of 24 BALB/c mice were randomly and equally divided into 4 groups to be topically treated with ovalbumin （OVA group）, SEB （SEB group）, OVA ＋ SEB （OVA ＋ SEB group） and sodium chloride physiological solution（control group） respectively, so as to establish mouse models of epicutaneously induced AD-like skin inflammation. The AD-like skin lesions were evaluated by clinical observation and eczema area and severity index （EASI）. Biopsy specimens were obtained from lesional skin of mice and then subjected to toluidine blue staining and immunohistochemical staining to count the mast cells, observe the morphology and distribution of mast cells, and calculate the percentage of degranulated mast cells. Results After 7-week treatment, the OVA group, SEB group and OVA ＋ SEB group all showed severer local skin inflammation, higher EASI scores and denser infiltration of inflammatory cells compared with the control group. Moreover, the OVA ＋ SEB group showed significantly severer local skin inflammation, skin lesions and degree of infiltration of inflammatory cells compared with the OVA group and SEB group （all P 〈 0.05）. The number of mast cells in the dermis of AD-like skin lesions per high-power field （× 400） was significantly higher in the OVA group （median [quartile range]： 10.625 [3.675]）, SEB group （11.000 [4.163]） and OVA ＋ SEB group （13.875 [8.813]） than that in the control group （5.925 [2.088], all P 〈 0.05）. The SEB group （71.083% ± 14.519%） and OVA ＋ SEB group （58.767% ± 16.978%） both showed significantly higher percentage of degranulated mast cells compared with the OVA group （24.050% ± 11.161%, both P 〈 0.05） and control group （23.617% ± 8.132%, both P 〈 0.05）. Bivariate correlation analysis showed that the number of mast cells in the skin lesions

关 键 词：皮炎 特应性 肥大细胞 金黄色葡萄球菌 肠毒素类 类胰蛋白酶类 卵白蛋白 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]