燕山-太行山区黑带食蚜蝇(双翅目：食蚜蝇科)谱系地理学分析  

Phylogeographical analysis of Episyrphus balteatus( Diptera: Syrphidae ) in Yanshan-Taihang Mountains Area

作　　者：白茜茜 潘昭 任国栋 

机构地区：[1]河北大学生命科学学院,河北省动物系统学与应用重点实验室,河北保定071002 [2]河北大学博物馆,河北保定071002

出　　处：《生态学杂志》2018年第1期157-163,共7页Chinese Journal of Ecology

基　　金：科技部科技基础性研究工作专项(2012FY111100)、河北省重点实验室运行项目(14967611D)、河北大学中西部提升综合实力专项基金和河北省森林有害生物调查项目资助.

摘　　要：为了解燕山-太行山区黑带食蚜蝇不同地理种群的遗传分化及其谱系地理格局,对河北、内蒙古、山西、河南等地样本的线粒体DNA-COI基因片段进行扩增和测序,成功得到36条黑带食蚜蝇COI基因序列,其片段长度为658 bp,平均A+T含量(69.9%)明显高于C+G含量(30.1%),没有插入和缺失现象。对这些COI基因序列进行分析,结果显示,雾灵山种群具有较高的种群遗传多样性。AMOVA分析表明,黑带食蚜蝇遗传分化主要来自种群内部。Mantel检验结果显示,各种群的遗传距离和地理距离之间相关性不显著(P=0.639),不符合地理隔离模型的推断。使用最大似然法构建系统发育树,结果表明:河北承德雾灵山种群中的单倍型Hap3独立为一支;对各种群的分歧时间进行估计,发现其分歧时间大约在0.02~0.94 Ma,处于更新世,部分种群间未发生分化。据此推测,燕山-太行山区黑带食蚜蝇的分化可能是由第四纪冰期气候的反复变化导致的,且雾灵山应为该地区黑带食蚜蝇在第四纪最后一次冰期的重要避难所之一。In order to understand the genetic differentiation and the phylogeographical structure ofEpisyrphus balteatus in Yanshan Taihang Mountains Area, a fragment with 658 bp from the mitochondria COI gene was extracted, expanded and sequenced for the specimens ofE. balteatus from Hebei, Inner Mongolia, Shanxi, Henan and adjacent places. In total, 36 sequences were obtained, the average content of A＋T （69.9%） was obviously higher than C＋G content （30.1%）, and there were no insertions or deletions. The results showed that the Wuling Mountain population had the highest genetic diversity. The analysis of molecular variance revealed the significant genetic subdivision among the individuals within populations. The result of Mantel test indicated that there was no significant correlation between the genetic distance and geographic distance （P=0.639）, so it did not conform to the inference of geographical isolation model. Phylogenetic analysis was constructed by using the maximum likelihood method, and results showed that the haplotype 3 from the Wuling Mountain population was well separated from others. The current study estimated the divergence time of populations, which was in the Pleistocene （0.02-0.94 Ma） and part of populations was without differentiation. We supposed that the reasons of differentiation ofE. balteatus in Yanshan-Taihang Mountains Area are the repeated changes of Quaternary glacial climate, and the Wuling Mountain should be one of the important refuges forE. balteatus in the last ice age of the Quaternary.

关 键 词：黑带食蚜蝇 燕山-太行山 COI 遗传分化 分歧时间 避难所 

分 类 号：Q968[生物学—昆虫学]