血管内皮生长因子165b对人肝癌HepG_2细胞生物学特性的影响  

Effect of VEGF165b on biological characteristics of human hepatocellular carcinoma HepG_2 cells

作　　者：赵燕颖[1] 王秀岩[2] 秦国涛[1] 孙远杰[1] 刘志忠[1] 李然伟[2] 

机构地区：[1]吉林大学第四医院消化内科,吉林长春130011 [2]吉林大学第二医院泌尿外科,吉林长春130041

出　　处：《吉林大学学报（医学版）》2018年第1期101-105,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省卫计委青年基金资助课题(2013Q024);吉林省科技厅自然科学基金资助课题(200905139)

摘　　要：目的:探讨血管内皮生长因子165b(VEGF165b)对人肝癌HepG_2细胞生物学特性的影响,并初步研究其作用机制。方法:HepG_2细胞分为空白组(仅加转染试剂)、对照组(转染阴性对照PcDNA3.0表达载体)和PcDNA-VEGF165b组(转染VEGF165b表达载体PcDNA-VEGF165b)。MTT法检测各组HepG_2细胞生存率,RT-PCR法和Western blotting法检测HepG_2细胞中VEGF165b和VEGF165 mRNA和蛋白表达水平,Transwell小室法检测HepG_2细胞迁移能力。结果:与空白组比较,对照组HepG_2细胞中VEGF165b和VEGF165mRNA和蛋白表达水平无明显变化(P>0.05)。与空白组比较,PcDNA-VEGF165b组细胞中VEGF165bmRNA和蛋白表达水平明显升高(P<0.05),VEGF165 mRNA和蛋白表达水平明显降低(P<0.05)。空白组和对照组细胞生存率比较差异无统计学意义(P>0.05);与空白组和对照组比较,PcDNAVEGF165b组细胞生存率有所降低,但差异无统计学意义(P>0.05)。细胞迁移实验,空白组和对照组细胞迁移率比较差异无统计学意义(P<0.05);与空白组和对照组比较,PcDNA-VEGF165b组HepG_2细胞迁移率明显降低(P<0.05)。结论:VEGF 165b能抑制VEGF165基因和蛋白的表达,VEGF165b对肝癌细胞增殖无明显影响,但可降低肝癌细胞的迁移能力。Objective:To study the effect of vascular endothelial growth factor 165 b(VEGF165 b)on the biological characteristics of human hepatocellular carcinoma HepG_2 cells,and to explore its mechanism.Methods:The HepG_2 cells were divided into blank group(treated with transfection reagent),control group(transfected with pcDNA3.0 expression vector)and pcDNA-VEGF165 b group(transfected with pcNDAVEGF165 b).MTT assay was used to detect the survival rate of HepG_2 cells;RT-PCR and Western blotting method were used to detect the expression levels of VEGF165 band VEGF165 mRNA and protein in the HepG_2 cells;Transwell chamber assay was used to measure the migration ability of HepG_2 cells.Results:Compared with blank group,there was no significant changes in the expression levels of VEGF165 band VEGF165 mRNA and protein in the HepG_2 cells in control group(P>0.05).Compared with blank group,the expression levels of VEGF165 bmRNA and protein in the HepG_2 cells in PcDNA-VEGF165 bgroup were significantly increased(P<0.05),and the expression levels of VEGF165 mRNA and protein were significantly decreased(P<0.05).There was no significant difference in the survival rates between blank group and control group(P>0.05).The survival rate of HepG_2 cells in PcDNA-VEGF165 bgroup was lower than those in blank group and control group,but the differences were not statistically significant(P>0.05).The cell migration experiment results showed that there was no significant difference in the migration rate between blank group and control group(P<0.05).Compared with blank group and control group,the migration rate of HepG_2 cells in PcDNA-VEGF165 b group was significantly reduced(P<0.05).Conclusion:VEGF165 bcan inhibit the expressions of VEGF165 mRNA and protein,and VEGF165 bhas no effect on the proliferation of hepatocellular carcinoma cells;but it can reduce the migration of hepatocellular carcinoma cells.

关 键 词：肝肿瘤 HEPG2细胞 血管内皮细胞生长因子165b 血管内皮细胞生长因子165 细胞增殖 细胞迁移 

分 类 号：R735.7[医药卫生—肿瘤]