长链非编码RNALnc-DQ在肝癌组织中的表达及其对SMMC7721细胞干性特征的影响  

Expression of Lnc-DQ in hepatocellular carcinoma and its role in regulating of the stemness characteristics of SMMC7721 cells

作　　者：林泽伟[1] 曾兵 叶会霖 程帝[3] 刘吉奎[1] 

机构地区：[1]北京大学深圳医院肝胆外科,广东深圳518036 [2广州医科大学附属第六医院胃肠外科,]广东清远511518 [3]中山大学孙逸仙纪念医院胆胰外科,广州510120

出　　处：《岭南现代临床外科》2017年第6期665-668,共4页Lingnan Modern Clinics in Surgery

基　　金：深圳市科技计划项目(JCYJ20150403091443331);广东省自然科学基金(2015A030310099);国家自然科学基金(81401996)

摘　　要：目的检测长链非编码RNA Lnc-DQ在肝癌组织中的表达,探讨其对肝癌SMMC7721细胞干性特征的影响。方法采用实时定量反转录聚合酶链反应(RT-q PCR)检测Lnc-DQ在30例肝癌组织和癌旁组织中的表达;sh RNA干扰下调Lnc-DQ在SMMC7721细胞中的表达,克隆形成和成球培养实验观察其对SMMC7721肿瘤干细胞特征的影响;流式细胞仪检测CD133+细胞亚群的比例。结果 Lnc-DQ在肝癌组织中的相对表达显著高于癌旁组织(3.24±0.34 vs1.81±0.17),差异具有统计学意义(P<0.05)。sh RNA转染可显著下调Lnc-DQ在SMMC7721细胞中的表达(P<0.05)。实验组细胞(sh-Lnc-DQ)克隆形成数为43.62±5.35,较对照组(sh-NC)显著减少(94.31±5.07),差异具有统计学意义(P<0.05)。成球培养实验显示sh-Lnc-DQ可显著抑制SMMC7721细胞的成球能力(P<0.05)。实验组细胞CD133+细胞比例显著低于对照组织(2.32%±0.25%vs 9.24%±0.69%),差异具有统计学意义(P<0.05)。结论 Lnc-DQ在肝癌中高表达,下调Lnc-DQ的表达能够抑制肝癌SMMC7721细胞的干性功能。Objective To investigate the expression of Lnc-DQ in hepatocellular carcinoma(HCC)and its role in regulating of the stemness characteristics of SMMC7721 cells. Methods The expression of Lnc-DQ was determined by real-time quantitative reverse transcriptase polymerase chain reaction(RT-q PCR)in HCC samples and cell lines. Cells were infected with negative control short hairpin RNAs(sh-NC)or specific sh RNAs targeting Lnc-DQ(sh-Lnc-DQ)to generate stable Lnc-DQ knockdown cell lines. Colon formation,anchorage-independent sphere formation and flow cytometry assays were used to analyze the effects of Lnc-DQ knockdown on the cancer stem-like phenotype of SMMC7721 cells. Results Lnc DQ was up-regulated in both HCC tissue samples and cell lines. sh RNA transfection could significantly reduced the expression of Lnc-DQ in HCC cells. For colon formation assay,the colony number was 43.62±5.35 in sh-Lnc-DQ group,and was significantly lower than 94.31±5.07 in sh-NC group(P<0.05). The mammosphere number in sh-Lnc-DQ group was small and lower than in sh-NC group(P<0.05). The proportion of CD133 positive was 2.32%±0.25% in sh-Lnc-DQ group and 9.24%±0.69% in sh-NC group(P<0.05). Conclusion Lnc DQ was up-regulated in HCC and play an important role in the regulating of the stemness related characteristics of SMMC7721 cells.

关 键 词：长链非编码RNA Lnc-DQ 肝癌 干性特征 

分 类 号：R735.7[医药卫生—肿瘤]