UFLC-QTRAP-MS/MS法同时测定绞股蓝中11种氨基酸  

Simultaneous determination of eleven amino acids in Gynostemma pentaphyllum by UFLC-QTRAP-MS/MS

作　　者：马赟[1] 蔡静[1] 张园娇 纪乐军 陈建伟[1] 李祥[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏神华药业有限公司,江苏淮安211600

出　　处：《中成药》2018年第1期133-137,共5页Chinese Traditional Patent Medicine

基　　金：江苏省科技成果转化项目(BA2014118);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的建立UFLC-QTRAP-MS/MS法同时测定绞股蓝Gynostemma pentaphyllum(Thunb.)Makino中苯丙氨酸、异亮氨酸、甲硫氨酸、缬氨酸、脯氨酸、酪氨酸、天冬氨酸、组氨酸、精氨酸、赖氨酸、谷氨酸的含有量。方法绞股蓝水提液的分析采用Waters XBridge Amide色谱柱(2.1 mm×100 mm,3.5μm);以水-乙腈为流动相(含0.2%甲酸),梯度洗脱;体积流量0.6 m L/min。结果 11种氨基酸在各自范围内线性关系良好(r>0.998 9),平均加样回收率90.74%~103.05%,RSD 0.61%~5.00%。结论该方法准确稳定,重复性好,可用于绞股蓝的质量控制。AIM To establish a UFLC-QTRAP-MS/MS method for the simultaneous content determination of phenylalanine, isoleucine, methionine, valine, proline, tyrosine, aspartic acid, histidine, arginine, lysine and glutamic acid in Gynostemma pentaphyllum （Thunb.） Makino. METHODS The analysis of aqueous extract of G. pentaphyllum was performed on a Waters XBridge Amide column （2. 1 mm × 100 mm, 3.5 μm） , with the mobile phase comprising of water-acetonitrile （ containing 0. 2% formic acid） flowing at 0.6 mL/min in a gradient elution manner. RESULTS Eleven amino acids showed good linear relationships within their own ranges （r 〉 0. 998 9）, whose average recoveries were 90. 74% - 103.05% with the RSDs of 0. 61% -5.00% . CONCLUSION This accurate, stable and reproducible method can be used for the quality control of G. pentaphyllum.

关 键 词：绞股蓝 氨基酸 UFLC-QTRAP-MS/MS 

分 类 号：R284.1[医药卫生—中药学]