SIRT-1及E-cadherin在胃癌组织中表达的相关研究  

Correlation study of expressions of silence signal regulating factor-1 and E-cadherin in gastric cancer

作　　者：李明辉[1] 董永杰[1] 赵静雅[2] 李玉梅[1] 冯运章[1] 霍双[3] 

机构地区：[1]邯郸市中心医院普外二科,河北邯郸056001 [2]邯郸市中心医院病理科,河北邯郸056001 [3]邯郸市中心医院科研科,河北邯郸056001

出　　处：《中国普外基础与临床杂志》2018年第1期42-47,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：河北省科技厅重点研发计划项目(项目编号:162777224)

摘　　要：目的探讨沉默信息调节因子-1(silence signal regulating factor-1,SIRT-1)和上皮型钙黏附素(epithelial cadherin,E-cadherin)在胃癌组织中的表达及其临床意义。方法采用免疫组织化学SP方法检测SIRT-1和E-cadherin蛋白在胃癌组织及其相应的癌旁组织中的表达情况,采用Spearman方法分析SIRT-1与E-cadherin蛋白表达的相关性。结果 SIRT-1蛋白表达阳性率在胃癌组织中明显高于其相应的癌旁组织(χ~2=5.791,P=0.016),其在弥漫型胃癌组织中明显低于肠型胃癌组织(P<0.05),而其阳性表达与胃癌患者的年龄、性别、肿瘤大小、肿瘤部位、分化程度、TNM分期及淋巴结转移均无关(P>0.05)。E-cadherin蛋白表达阳性率在胃癌组织中明显低于其相应的癌旁组织(χ~2=10.868,P=0.001),其在弥漫型胃癌组织中明显高于肠型胃癌组织(P<0.05);同样与胃癌患者的年龄、性别、肿瘤大小、肿瘤部位、分化程度、TNM分期及淋巴结转移均无关(P>0.05)。SIRT-1与E-cadherin蛋白在胃癌组织中的表达呈负相关关系(r_s=–0.381,P=0.013)。结论胃癌组织中SIRT-1的表达增高可能通过下调E-cadherin表达从而促使肿瘤的发生和发展,SIRT-1及E-cadherin的表达变化可能参与了肠型胃癌的发生。Objective To investigate expressions of silence signal regulating factor-1 （SIRT-1） and epithelial cadherin （E-cadherin） in gastric cancer and their clinical significances. Methods The immunohistochemistry SP technique was used to detect the expressions of SIRT-1 and E-cadherin in the gastric cancer tissues and their corresponding paracancerous gastric tissues. The relationship between the SIRT-1 expression and E-cadherin expression was analyzed using Spearman. Results The positive rate of the SIRT- 1 protein expression in the gastric cancer tissues was significantly higher than that of the corresponding paracancerous gastric tissues （x^2=5.791, P=0.016）. The SIRT-1 protein positive expression was related to the Lauren histological type of gastric cancer （x^2=4.941, P=0.026）, in other words, in the intestinal type was significantly higher than that of the diffuse type, but which was not related to the age, gender, tumor size, tumor site, differentiation degree, TNM stage, or lymph node metastasis （P〉0.05）. While the positive rate of the E-cadherin protein expression in the gastric cancer tissues was significantly lower than that of the corresponding paracancerous gastric tissues （x^2=10.868, P=0.001）, which in the intestinal type of gastric cancer was significantly lower than that in the diffuse type of gastric cancer （x^2=5.203, P=0.023）, also not related to the age, gender,tumor size, tumor site, differentiation degree, TNM stage, or lymph node metastasis （P〉0.05）. There was a negative correlation between the SIRT-1 protein and the E-cadherin protein （rs=-0.381, P=0.013）. Conclusions Gastric cancer with higher SIRT-1 expression might be way to achieve tumor development through E-cadherin as a facilitator. Upregulation of SIRT-1 and declining of E-cadherin might play a possible role in intestinal type gastric cancer.

关 键 词：胃癌 沉默信息调节因子-1 上皮型钙黏附素 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]