利用siRNA高通量测序技术检测平菇病毒  

Detection of Pleurotus ostreatus Virus by si RNA High-throughput Sequencing

作　　者：丛倩倩[1] 兰玉菲[1] 崔晓[1] 安秀荣[1] 

机构地区：[1]泰安市农业科学研究院,山东泰安271000

出　　处：《中国食用菌》2018年第1期61-64,共4页Edible Fungi of China

基　　金：国家现代农业(食用菌)产业体系建设专项(CARS-24);山东省食用菌创新团队病虫害防控与质量控制岗位(SDAIT-07-04)

摘　　要：为了检测采集于聊城市郑家镇的球形平菇(Pleurotus ostreatus)样品是否感染病毒,提取样品总RNA,构建小RNA文库并对其进行高通量测序,然后利用生物信息学方法寻找其中的病毒序列。结果证明球形平菇样品中未携带病毒,说明这种球形症状的产生不是由于病毒侵染所导致的。To identify virus in spherical Pleurotus ostreatus samples collected in Zhengjia town of Liaocheng city, we extracted the total RNA of the sample, constructed a small RNA library and made a high-throughput sequence, and then used the bioin- formatics methods to search for the virus sequence. The results showed that no virus genomic information was detected in col- lected mushroom samples, which suggested that the symptom was not caused by viral infection.

关 键 词：平菇 高通量测序 小干扰RNA 重叠群 

分 类 号：S646.1[农业科学—蔬菜学]