大孔吸附树脂分离纯化猪毛菜总黄酮及其抗氧化活性  

Separation and Purification of Total Flavonoids from Salsola collina with Macroporous Resins and Evaluation of Antioxidant Activities

作　　者：王沙沙 刘洋[1] 牛真真 公衍玲[1] 

机构地区：[1]青岛科技大学化工学院药学系

出　　处：《精细化工》2018年第1期58-64,共7页Fine Chemicals

基　　金：国家自然科学基金青年基金(81300281);山东省高校科技计划项目(J15LK12)~~

摘　　要：采用不同大孔吸附树脂分离纯化猪毛菜总黄酮,并对纯化后的总黄酮进行体外抗氧化活性测试。通过考察影响树脂静态和动态吸附与洗脱的主要因素,确定猪毛菜总黄酮分离纯化优化工艺条件。静态吸附实验表明,AB-8树脂分离纯化效果较好,并且吸附符合Langmuir和Freundlich方程。动态吸附和解吸的最佳工艺条件为：上样液质量浓度1.25 g/L、p H=4.5、上样流速2 m L/min、上样量2.5 BV（BV指树脂柱内装载树脂的体积）、洗脱剂为体积分数80%的乙醇溶液、洗脱流速1.0 m L/min,洗脱剂用量4 BV。所得洗脱液中黄酮质量分数从纯化前10.20%增加到纯化后51.89%,回收率为84.43%。体外实验表明,纯化后的黄酮可以清除羟自由基和超氧阴离子自由基,并有较好的还原力。纯化后的黄酮可以作为一种潜在的天然抗氧化剂。The separation and purification process of total flavonoids from Salsola collina was carried out with macroporous resins and the antioxidant activities in vitro of purified total flavonoids were studied. Critical factors affecting the static and dynamic adsorption and desorption of total flavonoids were selected and optimized. The static adsorption experiments indicated that the AB-8 macroporous resin was more effective than other maeroporius resins and its adsorption data were well fitted to the Langmuir and Freundlich isotherms The optimum conditions for dynamic adsorption and desorption were sample concentration 1.25 g/L, pH = 4. 5, sample loading rate 2 mL/min, sample loading amount of 2.5 BV （the volume of the resin bed） ,4 BV ＇of 80% ethanol solution （volume fraction） as the eluting solvent with an eluting rate of 1.0 mL/min. Under the above-mentioned conditions, the mass fraction of total flavonoids in the eluent increased from 10. 20% before purification to 51.89% after purification, and the recovery yield was 84. 43% The antioxidant tests revealed that the purified total flavonoids could scavenge hydroxyl and superoxide anion free radicals and Therefore, the purified total flavonoids from Salsola collina is

关 键 词：猪毛菜 总黄酮 大孔吸附树脂 纯化 抗氧化活性 中药现代化技术 

分 类 号：R284.2[医药卫生—中药学]