儿茶素抑制NF-κB-TSLP通路缓解过敏性哮喘小鼠炎症反应  

Catechin remits the inflammation in allergic asthma mice by suppressing NF-κB-TSLP signal pathway

作　　者：潘增烽 周园[1] 阮岩[2] 罗霞[1] 闫亚杰 周联[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学第一附属医院耳鼻咽喉科,广东广州510405 [3]广州中医药大学第一临床医学院,广东广州510405

出　　处：《中国药理学通报》2018年第2期207-212,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81373697);广东省自然科学基金资助项目(No 2015A030313365)

摘　　要：目的探讨儿茶素(catechin)对过敏性哮喘小鼠炎症反应的治疗作用及其机制。方法采用BALB/c♀小鼠建立鸡卵清蛋白(OVA)致敏和激发的过敏性哮喘模型,给药组分别给予儿茶素(75、150、300 mg·kg^(-1))干预。记录外周血白细胞分类及计数;HE染色观察肺组织病理学改变;ELISA法检测血清OVA特异性Ig E水平及肺组织TSLP、IL-5、IL^(-1)3的含量;Western blot检测肺组织NF-κB p65、IκB蛋白表达情况;免疫荧光检测NF-κB p65核转录情况。结果儿茶素可明显减少外周血白细胞总数,降低血清OVA特异性Ig E含量,减少肺组织中TSLP以及Th2型炎症因子IL-5、IL^(-1)3的含量,抑制NF-κB p65核转录。结论儿茶素可有效缓解OVA所致的过敏性哮喘小鼠的Th2型炎症反应,其作用可能是通过调控NF-κB通路,抑制TSLP表达而实现的。Aim To explore the mechanism of catechin in inhibiting Th2-driven inflammation responses of allergic asthma mice. Methods Female BALB/c mice were chosen to establish an allergic asthma model by sensitized and excited with OVA. Then,the administered group was intervened with catechin by series of dose( 75,150,300 mg · kg^(-1)). The proportion of peripheral leukocyte cells was analysed,and pathological changes in lung tissues were observed by HE dyeing. The levels of TSLP,IL-5,IL^(-1)3 in lung tissues and OVA specific Ig E in mouse serum were measured using ELISA kit. NF-κB p65,IκB protein expression levels in lung tissues were investigated using Western blot,and NF-κB p65 nuclear translocation was detected with immunofluorescent technique. Results Catechin could not only effectively reduce the proportion of peripheral leukocyte cells, but relieve the levels of TSLP,Th2 inflammatory factor IL-5 and IL^(-1)3,and the activation of NF-κB signal pathway in lung tissues of allergic asthma mice. Conclusions Catechin can effectively relieve Th2 inflammation in allergic asthma mice induced by allergen OVA. The possible mechanism may be that it could reduce expression of TSLP by inhibiting the NF-κB signal.

关 键 词：儿茶素 过敏性哮喘 胸腺基质淋巴生成素 Th2型炎症 抗炎 NF-ΚB 

分 类 号：R285.5[医药卫生—中药学]