放射性碘标记的抗肺癌单克隆抗体在载瘤裸鼠体内的代谢  

The Catabolism of Radioiodinated Anti-lung-cancer Monoclonal Antibodies in Tumor-bearing Nude Mice

作　　者：施绪保[1] 么崇正[1] 周前[1] 王世真[1] 

机构地区：[1]协和医院

出　　处：《中国医学科学院学报》1991年第6期422-427,共6页Acta Academiae Medicinae Sinicae

摘　　要：放射性碘标记的抗人肺癌单克隆抗体2E3和6DI注入载人肺癌移植瘤裸鼠体内,静脉血放射性-时间曲线符合二室开放模型;血放射性清除半减期为3.4d,表观分布容积636ml/kg;标记抗体在体内的脱碘率为18.6％,脱落的放射性碘绝大部分从尿排出。腹腔给药吸收快,半吸收期2.08h,生物利用度89％。^(125)I标记的单克隆抗体注射后1～3d,放射性主要分布在肿瘤、血、肝、脾中,其次为肺、肾、胃、小肠;此后,血和正常组织的放射性下降,肿瘤中的放射性较高。^(131)I标记的单克隆抗体能使移植瘤清晰显像。Nude mice bearing humor lung cancer xenografts were injected intravenously or intraperitoneally with a mixture of radioiodinated anti-lung-cancer monoclonal antibodies, 2E3 and 6Dl. The blood radioactivity versus time curve was fitted to a two-compartment open model with a 3.4 day blood radioactivity clearance half-life and a 636 ml/kg apparent distribution volume. Radioiodinated 2E3 and 6Dl given intraperitoneally were rapidly absorbed, with a 2.08 absorption half-life and 89% bioavailability. The highest radioactivity levels were found in the tumor, blood, liver and spleen 1-3 days after injection; next came the lung, kidney, stomach and intestine. The relative radioactivity increa- sed in the tumor as levels in blood and normal tissues decreased. The in vivo deiodination of radioiodinated 2E3 and 6Dl was about 18.6% and free radio-iodine was excreted in the urine.

关 键 词：肺肿瘤 单克隆抗体 碘标记 放射性 

分 类 号：R734.203[医药卫生—肿瘤]