慢性肾衰竭营养不良大鼠骨骼肌细胞凋亡及中药的干预实验  

Skeletal Muscle Cell Apoptosis in Chronic Renal Failure Rats with Malnutrition and Effects of Shenshuai Yangzhen Capsule

作　　者：邓聪[1,2] 魏连波 汪东涛[3] 鲁路[3] 龚艳媚[1] 张娥铿 

机构地区：[1]广州市第一人民医院南沙医院,广东广州511457 [2]广州中医药大学第二临床医学院,广东广州511457 [3]南方医科大学珠江医院中西医结合肾病中心国家中医药管理局肾病重点学科,广东广州510282

出　　处：《中华中医药学刊》2018年第1期53-56,I0006,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173457,81373808);广东省中医药局科研项目(20161182);广州市第三批优秀中医临床人才研修项目(GZYC03201602);广州市医药卫生科技项目(20142A011019)

摘　　要：目的：观察慢性肾衰竭（chronic renal failure,CRF）营养不良大鼠骨骼肌细胞凋亡情况,及中药肾衰养真胶囊对其的影响。方法：SD雄性大鼠,采用5/6肾切除同时予4%酪蛋白饮食制作CRF营养不良大鼠模型,随机分为正常组,模型组,中药组和西药组。治疗4周后,检测肌酐（Scr）、尿素氮（BUN）、白蛋白（Alb）、血红蛋白（Hb）,TUNEL法检测骨骼肌细胞凋亡率,Western Blot检测骨骼肌细胞凋亡蛋白酶3（Caspase-3）活性片段（Cleaved Caspase-3）、B淋巴细胞瘤-2基因相关蛋白（Bcl-2）、Bcl-2相关X蛋白（Bax）蛋白水平。结果：模型组大鼠骨骼肌细胞凋亡率明显增加（P〈0.01）,Cleaved Caspase-3和Bax表达均显著增加（P〈0.01）,Bcl-2表达显著下降（P〈0.01）。中药干预后,CRF营养不良大鼠骨骼肌细胞凋亡率显著减少（P〈0.01）,Cleaved Caspase-3和Bax表达均显著下降（P〈0.01）,Bcl-2表达显著增加（P〈0.01）,同时大鼠Alb、Hb显著升高（P〈0.01）。结论：Caspase-3依赖的细胞凋亡可能参与了CRF营养不良骨骼肌蛋白的降解,并诱导了骨骼肌萎缩。中药肾衰养真胶囊可能通过下调Cleaved Caspase-3和Bax蛋白表达,上调Bcl-2蛋白表达,抑制骨骼肌细胞凋亡,从而改善CRF营养不良。Objective： To investigate the effect of Shenshuai Yangzhen Capsule on skeletal muscle cell apoptosis in chronic renal failure（ CRF） rats with malnutrition（ MN）. Methods： Male SD rats of SPF grade were established into CRF-MN model by 5/6 nephrectomy and 4% casein diet. Rats successfully modeled were randomized into three groups.Group A wastreated with Shenshuai Yangzhen Capsule;group B treated with composite α-keto acid and Group C was untreated. Besides,a normal control group was set up. After being treated for 4 weeks,the expressions of Cleaved Caspase-3,Bcl-2 and Bax protein in skeletal muscle were measured by Western Blot. The skeletal muscle cell apoptosis was detected by TUNEL staining. Meantime,the renal function related indexes,including albumin（ Alb）,haemoglobin（Hb） were measured. Results： Compared with Group C,the condition of MN in Group A was significantly improve（P 〈0. 01）,showing decrease of skeletal muscle cell apoptosis（ P 〈 0. 01）,up-regulated Bcl-2 protein expression and down-regulated Cleaved Caspase-3 and Bcl-2 protein expressions in skeletal muscle（ P 〈 0. 01）,markedly improvement of renal function（ P 〈 0. 01）. Conclusion： Shenshuai Yangzhen Capsule can improve the MN condition in rats with CRF,which is possibly by the way of depressing Cleaved Caspase-3 and Bcl-2 protein expressions and upgrading Bcl-2 protein expression in skeletal muscle.

关 键 词：慢性肾衰竭 营养不良 细胞凋亡 中药 肾衰养真胶囊 

分 类 号：R285.5[医药卫生—中药学]