低氧预处理对氯化锂-匹鲁卡品致痫大鼠的保护作用  

The protective effect of hypoxia preconditioning on epilepsy induced by fithium - pilocarpine in rats

作　　者：高晨[1] 白洁[2] 雷鹏[4] 荔志云[4] 朱文霞[3] 王洪运[1] 王向阳[1] 

机构地区：[1]兰州军区兰州总医院安宁分院胸心脑外科,730070 [2]兰州军区兰州总医院安宁分院检验科,730070 [3]兰州军区兰州总医院安宁分院骨科,730070 [4]兰州军区兰州总医院神经外科,730050

出　　处：《中华实验外科杂志》2018年第1期111-115,共5页Chinese Journal of Experimental Surgery

摘　　要：目的观察间断低氧预处理（IHP）对氯化锂-匹鲁卡品（Li-pilo）致痫大鼠的脑保护作用。方法96只SD大鼠，随机分为对照组、癫痫组和4个间断低氧预处理＋癫痫组。对5组大鼠通过腹腔注射氯化锂-匹鲁卡品建立癫痫模型。记录240 min的发作行为学视频用于分析，并通过水迷宫实验测试大鼠认识功能，并检测大鼠海马CA1区神经元凋亡率，组织学分级（HG）及神经元密度（ND）。结果氯化锂-匹鲁卡品注射后50～150 min之间，癫痫发作的改良Racine评分达到峰值。间断低氧预处理＋3 d癫痫组大鼠平均峰值评分[（1.42±0.50）分，P＝0.007]明显低于Seizure组[（4.15±0.72）分]和间断低氧预处理＋1 d[（2.16±0.69）分，P＝0.028]，7 d[（2.26±0.53）分，P＝0.030]及14 d[（3.96±0.87）分]癫痫组。间断低氧预处理＋3 d癫痫组大鼠平均逃避潜伏期[（38.75±15.32） s，P＝0.012]明显短于癫痫组[（54.33±3.90） s, P＝0.011]和间断低氧预处理＋1 d[（44.83±11.68） s，P＝0.047]，7 d[（44.73±11.18） s, P＝0.045]，其神经元凋亡率和HG也较高。癫痫组大鼠平均目标象限停留时间百分比和ND值[（29.63±4.95）%；（35.25±8.37）个/HP）明显低于Control组[（41.13±19.09）%, P＝0.013；（110.35±9.87）个/HP, P＝0.000]，间断低氧预处理＋1 d癫痫组[（（36.13±13.97）%, P＝0.037；（65.31±7.25）个/HP, P＝0.007]，间断低氧预处理＋3 d癫痫组[（42.38±20.15）%, P＝0.011；（98.50±14.20）个/HP, P＝0.000]和间断低氧预处理＋7 d癫痫组[（33.94±8.75）%, P＝0.040；（54.80±11.68）个/HP, P＝0.010]。 结论IHP预处理5 d可以对癫痫模型大鼠提供明确的抗癫痫保护作用。Objective To investigate the brain protection of intermittent hypoxia preconditioning （IHP） on rats with seizures induced by lithium-pilocarpine （Li-pilo）.Methods96 rats were randomly divided into control group, seizure group and four IHP-seizure group. The animal model of epilepsy was established by Li-pilo intraperitoneal injection in seizure group and four IHP-seizure groups （Li-pilo was injected 1, 3, 7, or 14 days after a 5-day regimen of IHP）. Subsequently, 240 min video data of seizure behavior was tested, histological grade （HG） and neuronal density （ND） in rat hippocampal CA1 subfield via terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling （TUNEL） and Nissl’s staining. ResultsModified Racine scales of induced seizure peaked on average between 50-150 min after Li-pilo administration. The average peak score in IHP-3 d seizure group [（1.42±0.50） points, P=0.007] was significantly lower than seizure group [（4.15±0.72） points] and IHP-1 d [（2.16±0.69） points, P=0.028]; 7 d [（2.26±0.53） points, P=0.030] seizure group. The average escape latency in seizure rats [（54.33±3.90） s was significantly longer than Control group [（36.95±15.71） s, P=0.011], IHP-1 d seizure group [（44.83±11.68） s, P=0.047], IHP-3 d seizure group [（38.75±15.32） s, P=0.012] and IHP-7 d seizure group [（44.73±11.18） s, P=0.045]. The neuronal apoptosis rate and HG in seizure group were also higher than the other groups. The average percentage of time in the probe quadrant and ND value in seizure rats [（29.63±4.95）%; （35.25±8.37） cells/HP] were obviously lower than Control group [（41.13±19.09）%, P=0.013; （110.35±9.87） cells/HP, P=0.000], IHP-1 d seizure group [（36.13±13.97）%, P=0.037; （65.31±7.25） cells/HP, P=0.007], IHP-3 d seizure group [（42.38±20.15）%, P=0.011; （98.50±14.20） cells/HP, P=0.000] and IHP-7 d seizure group [（33.94±8.75）%, P=0.040; （54.80±11.68） cells/HP, P=0.010].ConclusionThe results

关 键 词：低氧预处理 癫痫 凋亡 神经保护 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]