贝伐单抗联合NP方案对肺癌移植瘤小鼠肿瘤生长和血管的影响  

Effect of Avastin combined with NP chemotherapy regime on tumor growth and blood vessels in mice bearing PC -9

作　　者：郑天亮[1] 何占锋[1] 赵松[1] 

机构地区：[1]郑州大学第一附属医院胸外科,450052

出　　处：《中华实验外科杂志》2018年第1期116-118,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察贝伐单抗联合NP化疗方案对肺癌移植瘤小鼠肿瘤生长和血管的影响。方法将60只PC-9荷瘤小鼠随机分为对照组、单抗组、化疗组和联合组，对照组给予等体积磷酸盐缓冲液（PBS），单抗组小鼠尾静脉注射贝伐单抗6 mg/kg，化疗组小鼠给予腹腔注射吉西他滨4 mg/kg和顺铂4 mg/kg，联合组小鼠同时给予贝伐单抗、吉西他滨和顺铂。自治疗起分析3组小鼠肿瘤生长、死亡率，并采用酶联免疫吸附试验（ELISA）法检测3组小鼠外周血中血管内皮生长因子（VEGF）的水平，采用免疫组织化学分析肿瘤组织中微血管密度。结果与对照组比较，单抗组、化疗组和联合组荷瘤小鼠肿瘤生长速度明显减缓（t＝2.091，P＝0.021；t＝2.103, P＝0.025）。而联合组小鼠PC-9肿瘤的生长速度较单抗组和化疗组更缓慢（t＝2.913，P＝0.010; t＝3.016, P＝0.009）。与对照组比较，单抗组、化疗组和联合组小鼠荷瘤后存活率明显延长（P＝0.011，P＝0.007）。而联合组荷瘤小鼠的生存率较单抗组和化疗组明显延长（P＝0.005、P＝0.002）。单抗组和联合组小鼠肿瘤组织中VEGF mRNA和蛋白质水平较对照组和化疗组明显下降（t＝6.193，P＝0.000; t＝6.693，P＝0.000；t＝10.198, P＝0.000; t＝11.183，P＝0.000）。联合治疗组和化疗组小鼠肿瘤组织中微血管密度较对照组和化疗组明显减少（t＝3.187，P＝0.000; t＝3.481，P＝0.000）。结论贝伐单抗联合NP化疗方案可显著抑制肿瘤血管形成、抑制肿瘤生长和延长荷瘤小鼠生存时间。Objective To explore the effects of Avastin combined with NP chemotherapy regime on tumor growth and blood vessels of mice bearing PC-9.MethodsSixty nude mice bearing human lung adenocarcinoma PC-9 xenografted tumor were randomly divided into control group, avastin group, chemotherapy group and combination group. Mice in Avastin group were treated with Avastin （4 mg/kg） by intraperitoneal injection. Mice in control group were treated with phosphate buffer （PBS） by intraperitoneal injection. Mice in chemotherapy group were treated with Gemcitabine and cisplatin （4 mg/kg） by intraperitoneal injection. Mice in combination group were treated with Avastin （4 mg/kg）, Gemcitabine and cisplatin （4 mg/kg） by intraperitoneal injection. After 30 days of treatment, tumor growth and survival rate among four groups were analyzed. Vascular endothelial growth factor （VEGF） mRNA and protein levels in four groups were analyzed real-time quantitative polymerase chain reaction （Real-time PCR） and enzyme linked immunosorbent assay （ELISA）. Tumor vessels density was analyzed by immunohistochemistry.ResultsAs compared with control group, tumor growth in avastin group, chemotherapy group and combination group was significantly inhibited and survival rate was significantly increased （t=2.091, P=0.021; t=2.103, P=0.025; P=0.011; P=0.007）. Tumor growth in combination group was significantly inhibited and survival rate was significantly increased as compared with that in avastin group and chemotherapy group （t=2.913, P=0.010; t=3.016, P=0.009; P=0.005; P=0.002）. VEGF mRNA and protein levels in avastin group and combination group were significantly decreased as compared with those in control group and chemotherapy group （t=6.193, P=0.000; t=6.693, P=0.000; t=10.198, P=0.000; t=11.183, P=0.000）. As compared with Avastin group and combination group, tumor vessel density in chemotherapy group and control group was significantly reduced （t=3.187, P=0.000; t=3.481, P=0.000）.ConclusionAvastin can sig

关 键 词：贝伐单抗 吉西他滨 顺铂 肿瘤血管 移植瘤 

分 类 号：R734.2[医药卫生—肿瘤]