胃癌组织中p53、PTEN蛋白表达与胃癌术后复发及预后的相关性研究  

Correlation between the expression of p53, PTEN protein and recurrence, survival of gastric cancer after gastrectomy

作　　者：郭天华[1] 赵琨[1] 庞世刚[1] 李波[1] 李延海[1] 

机构地区：[1]山东省滨州市中心医院胃肠外科,251700

出　　处：《中国实用医刊》2017年第23期23-26,共4页Chinese Journal of Practical Medicine

摘　　要：目的探讨胃癌（GO）组织中p53、PTEN蛋白表达与胃癌根治术后复发和预后的关系。方法应用组织芯片技术和免疫组织化学PV-6000两步法检测108例胃癌组织标本和30例癌旁组织及30例非胃癌胃组织中p53、PTEN蛋白的表达，结合临床病理指标及随访资料，分析其与肿瘤术后复发和预后的关系。结果p53蛋白表达与胃癌根治术后5年生存率相关（P=0．002），阳性表达组5年生存率低；PTEN蛋白与胃癌根治术后1年复发率（P=0．02）及5年生存率（P=0．022）相关，1年复发组阳性表达率低，PTEN阳性表达组术后5年生存率高。Cox回归多因素分析显示：p53与PTEN蛋白联合阳性表达是影响胃癌根治术后5年生存率的独立预后因素。结论p53、PTEN蛋白联合检测可以作为胃癌根治术后预测复发和生存率的指标。Objective To study the expressions of p53, PTEN in the tissues of gastric carcinoma （GC） and the correlation between them and recurrence, prognosis of GC patients after gastrectomy. Methods The expressions of p53, PTEN in 108 GC tissues, 30 pericarcinous tissue and 30 non gastric cancer tissue were examined by tissue microarray and two-step methods of PV-6000 of immunohistochem- istry, the correlations between their expressions and recurrence, prognosis were analyzed according to the clinical pathological data and follow-up data. Results The expression of p53 protein was related to the 5-year survival rate after radical resection of GC （ P = 0. 002 ）, and the 5-year survival rate of the patients with p53 positive expression was lower than that of patients with p53 negative expression; the expression of PTEN was related to the 1-year recurrence rate （ P = 0. 02 ） and 5-year survival rate （ P = 0. 022 ） , patients with recurrence in 1 year has low positive expression of PTEN, the 5-year survival rate of the patients with positive expression of PTEN was higher. Muhivariant Cox regression analysis showed that the positive expression of p53 and PTEN protein is an independent prognostic factor for the 5-year survival rate after radical resection of GC. Conclusions Combined detection of p53 and PTEN protein can be used as an index to predict recurrence and survival rate after radical gastrectomy.

关 键 词：胃癌 复发 预后 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]