HIF-1α、ERα和MMP-9在甲状腺乳头状癌中的表达及临床意义  

Expressions and clinical significances of HIF-1α,ERα and MMP-9 in papillary thyroid carcinoma

作　　者：田薇[1] 薛刚[1] 吉建敏 魏洁[1] 吴靖芳[2] 

机构地区：[1]河北北方学院附属第一医院耳鼻咽喉头颈外科,河北张家口075000 [2]河北北方学院附属第一医院形态学实验室,河北张家口075000

出　　处：《中国耳鼻咽喉颅底外科杂志》2017年第6期559-562,共4页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：张家口市科技攻关计划(1411043H;1611055H)

摘　　要：目的探讨乏氧因子-1α(hypoxia inducible factor-1α,HIF-1α)、雌激素受体α(estrogen receptorα,ERα)与基质金属蛋白酶-9(matrix metalloproteinases-9,MMP-9)在甲状腺乳头状癌中的表达及与临床因素的关系。方法采用免疫组化技术对88例乳头状癌及癌旁组织中HIF-1α、ERα和MMP-9的表达情况进行检测,分析三者与临床参数的关系。结果甲状腺乳头状癌(papillary thyroid carcinoma,PTC)组织中HIF-1α、ERα和MMP-9三者阳性率(70.45%,62.5%和84.1%)均高于癌旁组织(P<0.01);PTC组织中HIF-1α、ERα和MMP-9阳性率与淋巴结转移和临床分期有关。在淋巴结转移组和临床Ⅲ~Ⅳ期病例3者阳性率均高于无淋巴结转移组和临床Ⅰ~Ⅱ期病例(P<0.05或P<0.01);ERα阳性率在直径>2 cm的病例高于直径≤2 cm者(P<0.05);HIF-1α与ERα、HIF-1α与MMP-9阳性率呈正相关(均为P<0.01);ERα阳性率与MMP-9也呈正相关(P<0.01)。结论甲状腺乳头状癌中HIF-1α、ERα高表达可能通过MMP-9参与PTC的临床进程。Objective To exlpore the expressions and clinical signifieanees of hypoxia inducible factor-1 alpha （HIF-1α）, estrogen receptor alpha （ERa） and matrix metalloproteinases-9 （MMP-9） in papillary thyroid carcinoma （PTC）. Methods The expressions of above-mentioned factors in specimens of PTC and para-carcinoma thyroid tissues from 88 PTC patients were detected by immunohistoehemieal method （IHC）. And the relationships among clinical parameters and their expressions were analyzed. Results The positive rates of HIF-1α, ERa and MMP-9 proteins in PTC were 70.45 % , 62.5 % and 84.1% respectively, which were higher than those of the para-carcinoma tissues （ all P 〈 0. O1 ）. And their positive rates in the specimens with lymph node metastasis or at clinical stage Ⅲ-Ⅳ were higher than those without lymph node metastasis or at clinical stage Ⅰ～Ⅱ （ P 〈 0.05 or P 〈 0.01 ） , which indicated that their positive rates were related to the lymph node metastasis and clinical stage of PTC. The positive rate of ERcdn specimens with diameter more than 2 cm was higher than that in those with diameter equal to or less than 2 cm （ P 〈 0.05 ）. Their positive rates were positively correlated with each other （ all p 〈 0. 01 ）. Conclusion Enhanced expressions of HIF-1α and ERαmay participate in the clinical course of FFC via the high expression of MMP-9.

关 键 词：乏氧因子-1α 雌激素受体d 基质金属蛋白酶9 甲状腺乳头状癌 免疫组化 

分 类 号：R736.1[医药卫生—肿瘤]