TLR9-mRNA的表达与急性胰腺炎病人炎性因子变化的相关性研究  

Study on the relationship between the expression of toll-like receptor 9-mRNA and inflammatory factors in patients with acute pancreatitis

作　　者：杨宏达 刘志[1] 

机构地区：[1]中国医科大学附属第一医院急诊科,沈阳110001

出　　处：《临床外科杂志》2017年第12期923-925,共3页Journal of Clinical Surgery

摘　　要：目的探讨单核巨噬细胞TLR9-mTLR9-mRNA的表达与急性胰腺炎(AP)病人炎性因子变化的相关性。方法 24小时内被诊断为AP的病人40例,分别于第1,3,5天采集病人的EDTAK2抗凝静脉血,并检测胰弹性蛋白酶、促炎细胞因子和抗炎细胞因子。分离外周血单个核细胞,采用逆转录聚合酶链式反应对TLR9-mRNA进行检测,分析其5天内表达变化规律。结果所有AP病人在第1天、第3天、5天时单核巨噬细胞TLR9-mRNA对照组比较,差异有统计学意义(P<0.05)。AP病人外周血单核巨噬细胞TLR9-mRNA的改变与胰弹性蛋白酶、促炎细胞因子呈明显正相关,差异有统计学意义(P<0.05)。结论外周血单核巨噬细胞TLR9-mRNA的表达改变与AP病人炎性因子(胰弹性蛋白酶、促炎细胞因子)表达上调有相关性,TLR9可能介导AP的发生与发展。Objective To investigate the correlation between the expression of Toll-like receptor9（ TLR9）-mRNA and inflammatory factors in patients with acute pancreatitis（ AP）. Methods A total of 40 patients with AP diagnosed in our hospital from February 2016 to September 2016 in 24 h were enrolled. EDTAK2 anticoagulant venous blood was collected on day 1,3,5. And pancreatic elastase,proinflammatory cytokines and anti-inflammatory cytokines were detected. Peripheral blood monouclear cells were isolated and the mRNA expression of TLR9 was tested by polymerase chain reaction. The change of TLR9-mRNA in 5 days was analyzed. Results The level of TLR9-mRNA in peripheral blood mononuclear cells of patients with AP at 1,3,5 days were significantly higher than that in the control group,and the difference was statistically significant（ P〈 0. 05）. Changes of the TLR9-mRNA expression in peripheral blood mononuclear cells in patients with AP was positively correlated with the levels of pancreatic elastase and proinflammatory cytokines,and the difference was statistically significant（ P〈 0. 05）. Conclusion The expression of TLR9-mRNA in peripheral blood mononuclear macrophages was closely related to the up-regulation of inflammatory cytokines in AP patients,suggesting that TLR9 may mediate the occurrence and development of AP. To investigate the correlation between the expression of TLR9-m TLR9-mRNA and inflammatory factors in patients with AP. Methods A total of 40 patients with AP diagnosed in our hospital from February 2016 to September 2016 in 24 h were selected. EDTAK2 anticoagulant venous blood was collected on day 1,3,5. And Pancreatic elastase,proinflammatory cytokines and anti-inflammatory cytokines were also detected. EDTAK2 anticoagulant venous blood was collected,tested the above indicators and used them as the benchmark level of the corresponding indicators. Results The levels of TLR9-mRNA in peripheral blood mononuclear cells of patients with AP at 1,3,5 days were significantly higher than in the contr

关 键 词：急性胰腺炎 胰弹性蛋白酶 促炎细胞因子 抗炎细胞因子 单核巨噬细胞TLR9-mRNA 

分 类 号：R576[医药卫生—消化系统]