混合脐血间充质干细胞诱导人脑胶质瘤细胞凋亡的作用机制  

Effect of Mixed Umbilical Cord Blood-derived Mesenchymal Stem Cells in Inducing Apoptosis of C6 Glioma Cells

作　　者：王晓宁 焦红亮[2] 杜伟[2] 李建斌[3] 杨波[2] 

机构地区：[1]郑州大学附属郑州中心医院放疗科,河南郑州450007 [2]郑州大学第一附属医院神经外科,河南郑州450052 [3]河南省红十字血液中心,河南郑州450003

出　　处：《肿瘤基础与临床》2017年第6期465-467,共3页journal of basic and clinical oncology

基　　金：河南省教育厅高等学校重点科研资助项目(编号:16A320057;15A310011);河南省医学科技攻关资助项目(编号:201503020)

摘　　要：目的探讨混合脐血间充质干细胞(UCB-MSCs)体外抑制C6胶质瘤细胞增殖、诱导凋亡的可能机制,为混合UCB-MSCs体内治疗胶质瘤提供一定的实验基础和理论依据。方法采用免疫组化法检测混合UCB-MSCs对C6胶质瘤细胞Caspase-8、Bax、Bcl-2和Caspase-3蛋白表达的影响。结果混合UCB-MSCs对C6胶质瘤细胞有使凋亡蛋白Caspase-8、Bax、Caspase-3表达增高和抑凋亡蛋白Bcl-2表达降低的趋势。结论体外混合UCB-MSCs对C6胶质瘤细胞具有抑制增殖作用,而且这种抑制作用可能与诱导细胞凋亡相关。Objective To study the mechanisms of mixed umbilical cord blood-derived mesenchymal stem cells （UCB-MSCs） in inhibiting proliferation and inducing apoptosis of C6 glioma cells. Methods Caspase-8, Bax, Bcl- 2, Caspase-3 protein expressions of C6 glioma cells were analyzed by immunohistochemistry after UCB-MSCs. Results Mixed UCB-MSCs inhibited the proliferation of C6 glioma cells in vitro. Caspase-8, Bax and Caspase-3 protein expressions were increased, however, bcl-2 protein expression level was decreased. Conclusion Mixed UCB- MSCs could inhibit the proliferation of C6 glioma cells in vitro, which is related to induction of apoptosis of glioma cells.

关 键 词：脐血 间充质干细胞 细胞凋亡 

分 类 号：R739.41[医药卫生—肿瘤] R730.23[医药卫生—临床医学]