一株猪流感病毒的遗传进化分析及其致病力研究  

Genetic evolution analysis and pathogenicity of a swine influenza virus

作　　者：刘清政 王宏宇[1,2] 杜以军 郑乾坤[3] 李敏 彭涛 郭立辉[2] 陈智 丛晓燕[2] 陈蕾[2] 于江[2] 时建立[2] 李俊[2] 肖一红[1] 孙文博[2] 刘思当[1] 吴家强[2] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [3]得利斯集团有限公司,山东潍坊262216

出　　处：《中国预防兽医学报》2017年第12期1022-1025,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：山东省博士基金(BS2013SW022);国家重点研发计划(2016YFD0500201);泰山学者工程专项经费等资助

摘　　要：为研究猪流感病毒(Swine influenza virus,SIV)A/swine/Dezhou/37/2015(H1N1)株的遗传进化和致病力,本研究对该分离株的8个基因节段进行了遗传进化分析,并接种小鼠观察临床表现及检测其肺脏的病理变化情况。结果显示:分离株HA基因的裂解位点为PSIQSR↓G,无多个连续碱性氨基酸插入,为低致病性SIV;HA、M、NA节段处于欧洲类禽H1N1分支;NP、PA、PB1、PB2节段处于2009甲型H1N1分支;NS节段处于北美三源重排分支;对小鼠致病力研究显示该毒株可感染小鼠并引起其肺脏组织病理学变化,但对小鼠无致死性。以上结果表明该分离株为一株低致病性的新型三源重排SIV。本研究为山东地区SIV的流行特点及变异情况提供了实验依据。To study the genetic evolution and pathogenicity of the swine influenza virus （SIV） strain[A/swine/Dezhou/37/2015 （H1N1）], eight gene segments were analyzed. The result showed the HA segments cleavage site was PSIQSR J, G and there were no continuous basic amino acids insertion. HA, M, NA segments belonged to the European avian-like H1N1 branch; NP, PA, PBI, PB2 segments belonged to the 2009 pandemic H1N1 branch and NS segment belonged to the North American triple reassortant virus branch. The infection experiment with the BALB/cmice by A/swine/Dezhou/37/2015 （H1N1） showed the pulmonary histopathologic changes but nonlethal to mice. These results demonstrated that A/swine/Dezhou/37/2015（H1N1） was a novel low pathogenic triple reassortant SIV. This study provids the basis for the epidemiological characteristics and gene variations of SIV in Shandong.

关 键 词：猪流感 遗传进化分析 致病性 

分 类 号：S852.65[农业科学—基础兽医学]