不同干扰方式下松江湿地沉积物细菌群落结构特征  

Characteristics of bacterial communities in Songjiang wetland sediments subject to different disturbances

作　　者：李森森[1] 马大龙[1] 王璐璐[1] 李莹[1] 苑亚茹[1] 

机构地区：[1]哈尔滨师范大学地理科学学院,哈尔滨150025

出　　处：《应用与环境生物学报》2017年第6期1074-1080,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(41501065);黑龙江省自然科学基金项目(D2015002);中国博士后科学基金项目(2017M611381);黑龙江省普通本科高等学校青年创新人才培养计划项目(UNPYSCT-2017177)资助~~

摘　　要：松江湿地是我国面积最大的天然城市湿地,沉积物微生物对湿地环境变化响应敏感.为了解不同干扰方式下松江湿地细菌群落多样性变化特征,运用末端限制性片段长度多态性(T-RFLP)技术,对比分析4种干扰方式(农业、工业、旅游和保护)对沉积物细菌群落结构的影响.结果发现:片段长度为91.5 bp的T-RF普遍存在于6个湿地中,除呼兰河口(农业)湿地外,均为最优势菌;87.5 bp T-RF只存在于呼兰河口(农业)和阿什河(工业)湿地,且为呼兰河口(农业)湿地最优势菌(41.15%);93.5 bp T-RF出现在金河湾(旅游)、白鱼泡(旅游)、太阳岛(旅游)和滨江(保护)湿地.细菌多样性指数滨江(保护)湿地最高,呼兰河口(农业)湿地最低.序列比对表明这些优势菌群分别属于变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes).冗余分析(RDA)结果表明,呼兰河口湿地(农业)细菌群落受总氮(TN)影响较大;阿什河湿地(工业)细菌群落与总碳(TC)、总磷(TP)、TC/TN和p H具有较高的相关性;太阳岛湿地(旅游)受p H和TC/TN影响较大;而金河湾(旅游)、白鱼泡(旅游)和滨江(保护)湿地细菌群落与环境因子相关性较小.本研究表明不同干扰方式下松江湿地沉积物细菌群落特征及其影响因子存在显著差异,结果可为松江湿地生态系统的保护与恢复提供科学依据.Songjiang wetland is the largest natural, urban wetland in China, and its sedimentary microorganisms are sensitive to changes in the wetland environment. In order to investigate variations in bacterial community diversity in Songjiang wetland, the effect of four kinds of disturbances （agriculture, industry, tourism, and protection） on the sedimentary bacterial community structure was analyzed using a terminal restriction fragment length polymorphism （T-RFLP） technique. The study found that the 91.5 bp T-RF generally existed in six wetlands and was the predominant bacteria, except in the Hulan Estuary （agriculture） wetland. The 87.5 bp T-RF was only found in the Hulan Estuary （agriculture） and Ashi River （industry） wetlands, and it was the predominant bacteria （41.15%） in the Hulan Estuary （agriculture） wetland. The 93.5 bp T-RF appeared in the Jinhe Bay （tourism）, Baiyupao （tourism）, Sun Island （tourism）, and Binjiang （protection） wetlands. The above results show that there were significant variations in the bacterial community structure in wetlands subject to different disturbances. The highest bacterial diversity index appeared in Binjiang （protection） wetland, whereas the lowest emerged in the Hulan Estuary （agriculture） wetland. Sequence alignment revealed that the dominant bacteria belonged to Proteobacteria, Bacteroidetes, and Firmicutes. The results of a redundancy analysis （RDA） showed that the bacterial community in Hulan Estuary （agriculture） wetland was drastically affected by total nitrogen （TN）; the bacterial community in Ashi River （industry） wetland had a high correlation with total phosphorus （TP）, total carbon （TC）, TC/TN, and pH; and the bacterial community in Sun Island （tourism） wetland was heavily influenced by pH and TC/TN. The bacterial communities in Jinhe Bay （tourism）, Baiyupao （tourism）, and Binjiang （protection） wetlands were less affected by environmental factors. The results show that there we

关 键 词：松江湿地 T-RFLP 细菌群落 环境因子 

分 类 号：X172[环境科学与工程—环境科学]