miRNA-27b在骨肉瘤中的表达及其临床意义  

Expression and clinical significance of circulating microRNA-27b in patients with osteosarcoma

作　　者：张其川[1] 王素芳[2] 王云峰[1] 杜云飞[1] 付新生[1] 

机构地区：[1]新乡市中心医院骨2科,河南新乡453000 [2]新乡市中心医院消毒供应中心,河南新乡453000

出　　处：《临床与病理杂志》2017年第12期2633-2638,共6页Journal of Clinical and Pathological Research

摘　　要：目的:探讨微小RNA(miRNA)-27b在骨肉瘤中的表达情况及诊断和预后价值。方法:选取经病理证实为骨肉瘤的患者血样51例,年龄和性别相匹配的51例健康者血样作为对照组。采用实时定量聚合酶链反应(q RT-PCR)检测骨肉瘤患者及健康对照组血清miR-27b表达水平。结果:相较于健康对照组,骨肉瘤患者血清中miR-27b表达水平明显升高(P<0.001)。miR-27b的高水平表达与较大的肿瘤体积(P=0.008),较高的TNM分级(P=0.001)及远处转移(P=0.001)相关。受试者工作曲线(RO C)提示血清miR-27b水平对骨肉瘤患者具有诊断价值。曲线下面积(AUC)为0.863,其中敏感度为77.8%,特异度为86.7%。Kaplan-Meier曲线分析表明miR-27b高水平表达组5年生存率较低。此外,单变量和多变量Cox回归分析表明miR-27b是骨肉瘤患者预后的一个独立危险因素(P<0.001和P=0.006)。结论:血清miR-27b水平可能成为骨肉瘤患者诊断及判断预后的生物标志物。Objective： To investigate the application of serum miR-27b in diagnosis and prognosis of patients with osteosarcoma. Methods： Using quantitative reverse transcription polymerase chain reaction （qRT-PCR） technique, we assessed expression of studied miR-27b in serum samples of 51 pairs of osteosarcoma patients and corresponding healthy individuals. Results： Serum levels of miR-27b were found to be significantly higher in osteosarcoma patients than those in healthy controls （P〈0.001）. Moreover, elevated serum levels of miR-27b in osteosarcoma patients were correlated with the large tumor size （P=0.008）, advanced TNM stages （P=0.001） and distant metastasis （P=0.001）. Receiver operating characteristic （ROC） curve analyses suggested that serum miR-27b had significant diagnostic value for osteosarcoma patients. It yielded an area under the curve （AUC） of ROC of 0.863 with 77.8% sensitivity and 86.7% specificity in distinguishing osteosarcoma patients from control subjects. Furthermore, Kaplan-Meier curve analysis indicated that osteosarcoma patients with a high serum miR-27b level had a significantly shortened overall survival （P〈0.001）. In addition, univariate and multivariable Cox regression analysis revealed that serum miR-27b level was an independent risk factor for overall survival （P〈0.001 and P=0.006, respectively）. Conclusion： Therefore, serum levels of miR-27b may serve as a potential non-invasive biomarker for diagnosis and prognosis of osteosarcoma patients.

关 键 词：miR-27b 骨肉瘤 诊断 预后 生物标志物 

分 类 号：R738.1[医药卫生—肿瘤]