结肠癌患者SIRT6表达与病理特征的相关性  

The correlation between SIRT6 expression and pathological features in patients with colon cancer

作　　者：耿长辉[1] 张春玲[2] 曹珍珍[3] 衣洪天 何苗[4] 黄树民[5] 菅晶晶 高萍[5] Geng Changhui;Zhang ChunLing;Cao Zhenzhen;Yi Hongtian;He Miao;Huang Shumin;Jian Jingjing;Gao Ping(Department of General Surgery, the Fifth Affiliated Hospital of Harbin Medical University, Daqing 163001, China;Department of Ultrasound, Daqing Oilfield General Hospital, Daqing 163001, China;Department of Cinical Laboratory, the Fifth Affliated Hospital of Harbin Medical University, Daqing 163001, China;Department of Pathology, Daqing Oil Field General Hospital, Daqing 163001, China;Department of Gastroenterology, Daqing Oil Field General Hospital, Daqing 163001, China)

机构地区：[1]哈尔滨医科大学附属第五医院普外科,大庆163001 [2]大庆油田总医院超声医学科,163001 [3]哈尔滨医科大学附属第五医院检验科,大庆163001 [4]大庆油田总医院病理科,163001 [5]大庆油田总医院消化内科,163001

出　　处：《中华结直肠疾病电子杂志》2018年第1期52-56,共5页Chinese Journal of Colorectal Diseases(Electronic Edition)

基　　金：大庆市指导性科技项目计划(No.ZDY-2016-029)

摘　　要：目的研究结肠癌患者SIRT6表达与病理特征的相关性及其可能的机制。方法纳入2015年4月至2017年4月哈尔滨医科大学附属第五医院普外科60例结肠癌患者作为研究对象,所有患者均取手术切除的结肠癌组织和癌旁正常结肠组织作为病检标本,采用Western blot法和免疫组化法检测SIRT6蛋白和HIF-1α表达水平,收集60例患者性别、年龄、肿瘤TNM分期、分化等临床病理特征,记录结肠癌和癌旁组织中SIRT6和HIF-1α水平,比较SIRT6不同表达水平病理特征的差异,分析SIRT6与病理特征和HIF-1α的关系。结果 Western blot法检测的结肠癌组织中SIRT6和HIF-1α蛋白表达分别为(1.87±0.65)和(1.72±0.59),癌旁组织中SIRT6和HIF-1α分别为(2.34±1.13)和(0.84±0.36),结肠癌组织和癌旁组织中SIRT6和HIF-1α表达水平差异有统计学意义(t=2.793,9.862;P=0.006,0.000)。60例患者中结肠癌组织中SIRT6蛋白阳性13例,阴性47例。Logistic多因素分析显示肿瘤分期是影响结肠癌患者癌组织中SIRT6蛋白高表达水平的独立因素(OR=0.335;P=0.000)。结肠癌组织中SIRT6蛋白表达与HIF-1α水平呈显著负相关性(r=-0.819,P=0.000)。结论结肠癌组织中SIRT6蛋白表达水平与肿瘤分期关系密切,SIRT6蛋白对结肠癌病理进程有抑制作用,这可能与SIRT6调控HIF-1α蛋白有关。ObjectiveTo study the correlation between SIRT6 expression and pathological features in patients with colon cancer and its possible mechanism. MethodsSixty patients with colon cancer from April 2015 to April 2017 in Department of General Surgery, Fifth Af？liated Hospital of Harbin Medical University were included in this study, all colon cancer tissues and para cancerous normal colon tissues by surgical excision were taken as pathological specimens.The expression levels of SIRT6 protein and HIF-1α were detected by Western blot method and immunohistochemical method.The clinical and pathological characteristics of gender, age, tumor TNM stage and differentiation in 60 patients were collected. The levels of SIRT6 and HIF-1α in colon cancer and paracancerous tissues were recorded. The difference of pathological features of SIRT6 with different expression levels were compared. The relationship between SIRT6 and pathological features and HIF-1α were analyzed. ResultsThe protein expressions of SIRT6 and HIF-1α in colon cancer tissues by western blot were （1.87±0.65） and （1.72±0.59）, the levels of SIRT6 and HIF-1α in the para cancerous normal colon tissues were （2.34±1.13） and （0.84±0.36）. There were significant difference of the expression of SIRT6 and HIF-1α between colon cancer tissues and the para cancerous normal colon tissues （t=2.793, 9.862; P=0.006, 0.000）.The positive SIRT6 protein were 13 cases and the negative were 47 cases among the 60 patients.The logistic multivariate analysis showed that tumor staging was the independent factor, which affected the high expression of SIRT6 protein in colon cancer tissues （OR=0.335; P=0.000）. The expression of SIRT6 protein was negatively correlated with the level of HIF-1α in colon cancer tissues （r=-0.819, P=0.000）. ConclusionThe expression of SIRT6 protein in colon cancer tissues are closely related to tumor staging.The SIRT6 protein may inhibit the pathological process of colon cancer, which may be related to the regulation

关 键 词：结肠肿瘤 SIRT6 病理特征 相关性 

分 类 号：R735.35[医药卫生—肿瘤]