GC-MS技术分析尤力克柠檬花挥发油的化学成分  

Analyze Chemical Constituents of Eureka Lemon Flower Essential Oil by GC-MS

作　　者：金玲[1] 杜鸿灵 蒋泽林 罗国军 吴文林 张旭[1] 

机构地区：[1]成都中医药大学药学院,教育部中药材标准化重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137 [2]隆昌重峻农业有限公司,四川隆昌642150 [3]成都市食品药品检验研究院,成都610100

出　　处：《中国实验方剂学杂志》2018年第3期56-61,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省科技厅计划项目(2017NFP0158)

摘　　要：目的：对尤力克柠檬花的挥发油成分进行分析鉴定,为柠檬花开发利用提供科学依据,从而有效利用柠檬资源,促进柠檬产业链的发展。方法：采用水蒸气蒸馏法提取柠檬花中的挥发油并计算收率,以GC-MS结合NIST14.0质谱库、保留指数和文献资料共同分析鉴定挥发油化学成分及种类,用峰面积归一化法计算各成分的相对百分含量。结果：尤力克柠檬花中挥发油的提取率为0.36%,共分离出76个峰,鉴定出64个成分,占挥发油总量的98.94%。柠檬花中挥发油化学成分主要有烯烃类（28种）占81.90%,醇类（14种）占12.28%,醛类（6种）占3.34%,酯类（5种）占0.45%,烷烃类（6种）占0.79%;其中,含量由高到低的有d-柠檬烯（59.88%）,β-蒎烯（5.52%）,顺-β-罗勒烯（5.33%）,反-橙花叔醇（4.84%）,3,7,11-三甲基-6,10-十二烯-1-醇（4.49%）和γ-萜品烯（4.11%）等。结论：柠檬花挥发油化学成分绝大部分与柠檬果皮中的相似,均含有d-柠檬烯,β-蒎烯等活性成分,且d-柠檬烯含量（59.88%）远高于文献报道柠檬叶中d-柠檬烯含量（17.22%）,这意味着尤力克柠檬花具有潜在开发利用价值。Objective： To provide scientific evidence for development and utilization of Eureka lemon flower so as to effectively make use of lemon and promote the industry chain by analyzing and identifying the chemical constituents of essential oil by GC-MS. Method： The essential oil of Eureka lemon flower was extracted by steam distillation and the oil yield was calculated. The oil sample was analyzed by GC-MS,and the chemical components were identified by combining mass NIST 14. 0 spectrometry database with retention index（RI） and documents,then the peak area normalization method was used to calculate the relative percentage of each ingredient. Result： The essential oil yield was 0. 36%. A total of 76 components were detected and 64 components were identified,accounting for 98. 94% of the total constituents. The chemical compositions from lemon flower volatile oil mainly included olefin（28） 81. 90%,alcohol（14） 12. 28%,aldehydes（6） 3. 34%,esters（5）0. 45%,and alkane（6） 0. 79%, etc. Its major components included d-limonene（59. 88%）, β-pinene（5. 52%）,（Z）-β-ocimene（5. 33%）,trans-nerolidol（4. 84%）,3,7,11-trimethyl-6,10-dodecadien-1-ol（4. 49%）,γ-terpinene（4. 11%）,etc. Conclusion： Most components of flower essential oil were similar to those in the leaves,which both include d-limonene,β-pinene and other active constituents. Beside that,the content of d-limonene（59. 88%） was much higher than that in the literature of lemon leaves（17. 22%）,indicating that Eureka lemon flowers have potential exploitation value.

关 键 词：尤力克柠檬 花 挥发油 气相色谱-质谱 化学成分 

分 类 号：R284[医药卫生—中药学] R282[医药卫生—中医学]