RNA结合蛋白Larp1在卵巢癌侵袭转移中的作用及机制  

Role of RNA-binding protein Larp1 in ovarian cancer invasion and metastasis and mechanism

作　　者：戴燕[1] 金世光[1] 王薛洁 杨鑫泉 王大新[1] 

机构地区：[1]江苏省苏北人民医院医学实验研究中心,江苏扬州225001 [2]江苏省苏北人民医院急诊科,江苏扬州225001

出　　处：《实用医学杂志》2018年第1期21-25,共5页The Journal of Practical Medicine

基　　金：江苏省苏北人民医院院基金(编号:yzucms201503);扬州市自然科学基金面上项目(编号:Yz2015093)

摘　　要：目的 RNA结合蛋白Larp1在卵巢癌细胞转移与上皮间质转化(EMT)中有重要作用,进一步探讨Larp1在上皮性卵巢癌细胞中的侵袭转移作用与机制具有重要临床意义。方法在卵巢癌细胞中通过慢病毒转染过表达Larp1,通过3D侵袭实验、定点趋化实验分析在促生长因子影响下Larp1过表达对细胞侵袭转移的影响。结果细胞质内Larp1表达及分布与卵巢癌侵袭转移能力呈正相关,Larp1过表达会促进卵巢癌EMT,增强卵巢癌细胞侵袭能力;Larp1过表达的SKOV-3与OVCAR-3细胞对促生长因子(TGF-β、EGF、b FGF)趋化反应更加明显;Larp1过表达能促进卵巢癌细胞肌动蛋白纤维与应力纤维生成且Larp1与肌动蛋白纤维共定位。结论卵巢癌细胞中Larp1过表达能促进癌细胞侵袭转移,因此更加全面地理解Larp1在卵巢癌中的作用,可为诊断卵巢肿瘤分期及治疗提供新的理论依据。Objective Recent evidence has implicated Larpl, an RNA-binding protein, in cell motility and EMT therefore prompting the study of Larpl in EOC. This project aims to examine the potential role of Larpl in cell invasion. Methods Ovarian cancer cells SKOV-3 and OVCAR-3 were transfected with DNA constructs to overexpress Larpl and transfect cells were used to assess the overexpression of Larpl in cell invasion and metasta- sis using several functional assay. Results Larpl overexpression in both OVCAR-3 and SKOV-3 cells appear to enhance the invasive ability of cells and the accumulation of Larp 1 in these ehemoinvasive protrusions strongly sug- gests the potential role of Larpl in invadopodia formation. Cell spot chemoinvasion assay demonstrated increased chemoinvasion of Larpl overexpressing SKOV-3 and OVCAR-3 cells towards a gradient of TGF-β, EGF and bFGF. Conclusions With its potential role in EMT and cell invasion, a deeper understanding of the physiological role of Larpl in cancer cell motility will be potentially beneficial in the diagnosis and treatments of ovarian cancer.

关 键 词：Larp1 卵巢癌 上皮间质转化 侵袭与转移 

分 类 号：R737.31[医药卫生—肿瘤]