双波长分光光度法测定杨梅叶中总黄酮的研究  

Assaying Total Flavonoids in Myrica rubra Leaves by Double-wavelength Spectrophotometry

作　　者：辜忠春[1,2] 李光荣 洪海彦[3] 王宵 李晖 马林江[1] 

机构地区：[1]湖北省林业科学研究院,武汉430075 [2]国家林业局林产品质量检验检测中心,武汉,430075 [3]洪湖市林业科学研究所,荆州433200

出　　处：《食品工业》2018年第1期295-297,共3页The Food Industry

基　　金：湖北省林业科技支撑重点项目([2011]3801)

摘　　要：以超声波法辅助乙醇提取杨梅叶试样中的总黄酮,采用双波长分光光度法测定提取液的黄酮浓度。结果表明,在测定波长415 nm,参比波长475 nm条件下,芦丁标准品的标准曲线为ΔA=0.025 9c+0.002 5,芦丁标准品的质量浓度在3.7~37.1 mg/L与ΔA呈良好的线性关系(R^2=0.999 7),检出限为0.1 mg/g,测定结果的相对标准偏差(n=6)为2.46%,加标回收率在95.27%~101.35%,建立的试验方法具有良好的准确度及精密度。The total flavonoids of Myrica rubra leaves were determined by double-wavelength spectrophotometry. The Myrica rubra leaves were degreased by ethyl ether, and the total flavonoids were extracted by ultrasonic-assisted method. The detective wavelength was 415 nm; The reference one was 475 nm; The rutin standard curve was AA=0.025 9c＋0.002 5; A good liner relationship （R2=0.999 7） was obtained in 3.7-37.1 mg/L mass concentration. The detection limit of the method was 0.1 mg/g; The relative standard deviation （n=6） was 2.46%; The recovery of standard addition was 95.27%-101.35%. The established test method had good accuracy and orecision.

关 键 词：总黄酮 双波长分光光度法 杨梅叶 芦丁 

分 类 号：O657.3[理学—分析化学] R284.1[理学—化学]