长链非编码RNA LINC00520在喉鳞状细胞癌中的表达与临床意义  

Expression and clinical significance of long non-coding RNA LINC00520 in laryngeal squamous cell carcinoma

作　　者：吴勇延[1] 高伟[1] 张宇良 牛敏[1] 崔佳佳 相彩霞 桑江玮 温树信[1] 王斌全[1] 

机构地区：[1]山西医科大学第一医院耳鼻咽喉头颈外科,太原030001

出　　处：《临床耳鼻咽喉头颈外科杂志》2018年第2期91-95,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：中国博士后科学基金(No:2016M591412;No:2017M610174);山西省头颈部恶性肿瘤精准诊疗重点科技创新平台专项基金(No:2016025D151003);山西省科技成果转化引导专项(No:201604D131002;No:201604D132040);山西医科大学博士启动基金(No:BS03201624)

摘　　要：目的:研究长链非编码RNA LINC00520在喉鳞状细胞癌中的表达及与临床意义。方法:使用实时荧光定量PCR技术检测LINC00520在65例喉鳞状细胞癌组织及癌旁正常黏膜组织中的表达情况,分析LINC00520表达水平与患者临床分型、组织分化、淋巴结转移、临床分期等临床病理参数的关系。结果:喉鳞状细胞癌LINC00520表达水平显著高于癌旁正常黏膜组织(P<0.000 1);结合患者临床病理参数分析发现,声门型、声门上型和声门下型喉鳞状细胞癌LINC00520表达水平组间比较均差异无统计学意义(P>0.05)。低分化组喉鳞状细胞癌中LINC00520表达水平与高、中分化组相比,差异无统计学意义(P>0.05)。T1+T2组喉鳞状细胞癌与T3+T4组相比,LINC00520表达亦差异无统计学意义(P>0.05)。无淋巴结转移组喉鳞状细胞癌LINC00520表达水平显著低于淋巴结转移组(P<0.01)。结论:LINC00520表达上调可能是喉鳞状细胞癌转移中的重要分子事件并发挥重要生物学功能。Objective：To investigate the expression of LINC00520 in laryngeal squamous cell carcinoma（LSCC）,and analyze its relevance and roles in carcinogenesis and development of LSCC.Method：The expression of LINC00520 in laryngeal squamous cell carcinoma tissue and paired adjacent normal tissue was determined by real-time PCR.The relationship between the expression of LINC00520 and the clinicopathological characteristics including clinical stage,pathological type,histological grade and lymph node metastasis of LSCC was analyzed.Result：（1）The LINC00520 expression level was significantly upregulated in LSCC tissues compared to that of paired adjacent normal tissues（P0.000 1）.（2）There were no statistical differences of the LINC00520 expression level among supraglottic,glottic and subglottic LSCCs（P0.05）.The LINC00520 expression level had no significant changes in poorly differentiated LSCC compared with that of well and moderately differentiated counterparts（P0.05）.Moreover,the expression of LINC00520 had no significant difference between T1＋T2 stage and T3＋T4 stage LSCC tissues（P0.05）.Interestingly,the LINC00520 level in LSCC with lymph node metastasis was significantly higher than that in patients without lymph node metastasis（P0.01）.Conclusion：Upregulation of LINC00520 in LSCC may contribute to its metastasis.

关 键 词：长链非编码RNA LINC00520 喉肿瘤 肿瘤转移 

分 类 号：R739.65[医药卫生—肿瘤]