啤酒花中黄酮类成分的含量分析  

Analysis of flavonoids contents of hops

作　　者：李常惠 许月明[3,2] 张巧艳[2] 韩婷 辛海量[2] LI Changhui;XU Yueming;ZHANG Qiaoyan;HAN Ting;XIN Hailiang(School of Pharmacy , Shandong Univer-sity of Traditional Chinese Medicine , Jinan 250355 , C h i n a;Sch o o l of P h a r m a c y , Jiamusi University , Jiamusi 1 5 4 0 0 7 ,C h i n a;School of Pharmacy , Second Military Med i c a l University , S h a n g h a i 2 0 0 4 3 3 , C h i n a)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]第二军医大学药学院,上海200433 [3]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《药学实践杂志》2018年第1期64-67,共4页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金(U1603283)

摘　　要：目的建立啤酒花中总黄酮和黄腐酚含量测定方法,对来自不同产地和品种的29个啤酒花样品中的总黄酮和黄腐酚进行测定。方法用芦丁-AlCl3分光光度法测定总黄酮的含量;用HPLC-UV法测定黄腐酚的含量。色谱条件:色谱柱为Dikma Technologies Diamonsil C18柱(250mm×4.6mm,5μm);流动相为乙腈-1%冰醋酸梯度洗脱,流速为1.0ml/min;柱温为25℃;检测波长为370nm。结果总黄酮回归方程为A=30.345C+0.016 8,r=0.999 9;黄腐酚回归方程为A=55 446C+9 040.5,r=0.999 9,表明总黄酮在20.2~404.0μg/ml,黄腐酚在2.152~43.040μg/ml范围内,线性关系良好,两者精密度和重复性RSD均<2%,平均加样回收率分别为102.71%和100.21%。结论不同种类的啤酒花之间,总黄酮和黄腐酚含量存在巨大差异,进口啤酒花优于国产。Objective To determine the contents of total flavonoids and xanthohumol in hop from 29 different countries and regions.Methods Rutin colorimetric method was used to determine the content of total flavonoids.HPLC-UV method was established for the determination of xanthohumol in hops.HPLC method was performed by Dikma Technologies Diamonsil C18（250 mm×4.6 mm,5μm）column with mobile phase acetonitrile-1% glacial acetic acid solution at the flow rate of1.0 ml/min.The column temperature was 25 ℃.The detection wavelength was 370 nm.Results The equation of linear regression of total flavonoids was A=30.345 C＋0.016 8,r=0.999 9.The equation of linear regression of xanthohumol was A=55 446 C＋9040.5,r=0.999 9.Their linear ranges were respectively 20.2-404.0μg/ml,2.152-43.040μg/ml,which indicated a good linear relationship.The RSDs of precision and repeatability were less than 2%.The average recoveries of flavonoids and xanthohumol were respectively 102.71% and 100.21%.Conclusion The contents of total flavonoids and xanthohumol in different hops varieties are significantly different and the import hops was better than the domestic hops in this study.

关 键 词：啤酒花 总黄酮 黄腐酚 

分 类 号：R284.1[医药卫生—中药学]