雷公藤内酯醇对阿尔茨海默病大鼠海马drebrin和cofilin表达的影响  

Effects of Triptolide on Expression of Drebrin and Cofilin in Hippocampus of Rats with Alzheimer's Disease

作　　者：张赛圣[1,2] 杨宝林[1] 程丽霞 万斌[1] 聂菁[1] 胡小令[1] 吕诚 

机构地区：[1]南昌大学江西医学院,江西南昌市330006 [2]十堰市人民医院,湖北十堰市442000 [3]湖北省东风公司总医院,湖北十堰市442008

出　　处：《中国康复理论与实践》2018年第1期23-28,共6页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(No.81660191;No.30660073); 江西省教育厅科研基金项目(No.GJJ150105); 江西省卫生计生委科技计划项目(No.20165532)

摘　　要：目的观察雷公藤内酯醇对阿尔茨海默病(AD)模型大鼠海马神经元drebrin和cofilin表达的影响。方法雄性Sprague-Dawley大鼠60只随机等分成对照组(n=20)、模型组(n=20)和用药组(n=20)。大鼠右侧海马内注射β淀粉样蛋白(Aβ_(1-40))造模,对照组右侧海马内注射等量生理盐水。用药组在右侧海马注射Aβ_(1-40)后每天腹腔注射雷公藤内酯醇0.4 mg/kg,共15 d。Golgi染色检测各组海马神经元树突棘密度的变化。免疫组化染色和逆转录聚合酶链反应(RT-PCR)检测各组海马神经元drebrin和cofilin表达的变化。结果用药组大鼠海马神经元树突棘密度高于模型组(P<0.05)。用药组大鼠海马drebrin阳性产物平均光密度较模型组明显升高(P<0.01),cofilin阳性细胞数和平均光密度较模型组减少(P<0.05)。用药组大鼠海马drebrin mRNA表达高于模型组(P<0.05),cofilin m RNA表达明显低于模型组(P<0.01)。结论雷公藤内酯醇可能通过调节drebrin和cofilin的表达,延缓AD模型大鼠海马神经元树突棘的退化。Objective To observe the effects of triptolide on drebrin and cofilin expression in the hippocampus of rats with Alzheimer's disease(AD).Methods Sixty male Sprague-Dawley rats were equally divided into control group, model group and triptolide-treated group with 20 cases in each group. The AD model was established with unilateral injection of beta amyloid 1-40(Aβ_(1-40)) into hippocampus in rats. The control group was established with unilateral injection of normal saline with the same volume into hippocampus in rats. The triptolide-treated group was administered triptolide intraperitoneally, 0.4 mg/kg, once a day, for 15 days after modeling. Spine density of hippocampal neurons was assayed by Golgi staining. Drebrin and cofilin expression of hippocampal neurons was assayed by immunohistochemical staining and reverse transcription polymerase chain reaction(RT-PCR).Results The spine density of hippocampal neurons was higher in the triptolide-treated group than in the model group(P<0.05). The average optical density of drebrin was higher in the triptolide-treated group than in the the modelgroup(P<0.01), while the cell number and average optical density of cofilin were lower(P<0.05). The drebrin m RNA expression was higher in the triptolide-treated group than in the model group(P<0.05), and the cofilin m RNA expression was lower(P<0.01).Conclusion Triptolide may delay the degeneration of dendritic spines in hippocampal neurons of AD rats by regulating the expression of drebrin and cofilin.

关 键 词：阿尔茨海默病 雷公藤内酯醇 DREBRIN COFILIN 大鼠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]