蜂胶黄酮的乙醇分级提取及其抗氧化活性比较  

Graded ethanol method extraction and the antioxidant activity comparison of flavonoids from crude propolis

作　　者：姜红宇[1] 盘雪 李目武 王宗成[1,2] 

机构地区：[1]湖南科技学院湖南省银杏工程技术研究中心,湖南永州425100 [2]中南大学化学化工学院,湖南长沙410083

出　　处：《食品与机械》2017年第11期152-156,共5页Food and Machinery

基　　金：湖南省高校科技创新团队支持计划资助(编号:2012-318);湖南科技学院湘南优势植物资源综合利用湖南省重点实验室开放基金资助(编号:XNZW17C03);湖南科技学院科研重点项目(编号:17XKY009);湖南科技学院生物工程重点学科资助;永州市科技计划资助项目[编号:永科发(2016)27号-11]

摘　　要：为确定粗蜂胶中活性成分所在极性范围,选择乙醇分级提取永州粗蜂胶中的活性成分,以黄酮含量为指标,比较不同提取条件下蜂胶黄酮的含量差异,并测定不同提取条件下获得的提取物的抗氧化活性。结果表明:用40%~60%乙醇提取得到的蜂胶黄酮质量和含量较多;乙醇分级提取的提取物中蜂胶黄酮的含量可达到41.2%,黄酮得率可达25.81%;50%,60%乙醇提取的蜂胶黄酮抗氧化能力较强,活性高于Vc;30%乙醇提取蜂胶黄酮抗氧化能力最差,说明粗蜂胶中含有不同类型的蜂胶黄酮,提取活性较高的蜂胶黄酮,可以选择水提取水溶性黄酮和40%~70%乙醇提取中等极性黄酮。The active ingredients of crude propolis from Yongzhou were extracted by graded ethanol method and investigated the scope of their activity. With flavonoids content as index, content and the antioxidant activity of them in the crude propolis from different ex- traction conditions was evaluated, The results showed that the quality and content of flavonoids in the crude propolis extracted with 40%-60% ethanol were higher. The content of flavonoids in the ex- tracts by graded ethanol method reached 41.2% and the extraction yield of flavonoids was 25.81% . The antioxidant activity of flavonoids of crude propolis extracted with 50% and 60% ethanol were better than Vc, but the one in the crude propolis extracted with 30% etha- nol was the worst. It illustrated the crude propolis contained different types of flavonoids. To obtain high active flavonoids of crude propolis, water should be chosen as extraction solvent to water solu- ble flavonoids and 40% - 70% ethanol should be chosen as extraction solvent to medium polarity flavonoids.

关 键 词：蜂胶 黄酮 乙醇分级 抗氧化活性 

分 类 号：S896.6[农业科学—特种经济动物饲养] TQ28[农业科学—畜牧兽医]