miR-372-3p在膀胱癌组织中的表达及其对膀胱癌细胞增殖、迁移与侵袭的影响  

Expression of miR-372-3p in the tissues of bladder carcinoma and its effect on proliferation,migration and invasion of the bladder cancer cells

作　　者：张伟[1] 郭永连[1] 李国灏[1] 舒博 陈琳[1] 余家俊[1] 

机构地区：[1]华中科技大学同济医学院附属武汉中心医院泌尿外科,湖北武汉430014

出　　处：《中国肿瘤生物治疗杂志》2018年第1期45-50,共6页Chinese Journal of Cancer Biotherapy

摘　　要：目的:检测miR-372-3p在膀胱癌组织和转染miR-372-3p mimics膀胱癌细胞中的表达,研究miR-372-3p对膀胱癌5637和T24细胞增殖、迁移与侵袭的影响。方法:采集2016年3月至2017年1月武汉中心医院收治的6例膀胱癌患者癌及癌旁组织(距离肿瘤边缘>5 cm),q PCR检测膀胱癌及癌旁组织miR-372-3p表达。采用脂质体转染法将miR-372-3p mimics或者miRNC转入膀胱癌5637和T24细胞。用q PCR和Western blotting检测转染miR-372-3p mimics和miR-NC膀胱癌细胞miR-372-3p和ATAD2 m RNA和蛋白E-cadherin、N-cadherin蛋白的表达,流式细胞术检测细胞周期分布,MTT法和集落形成实验检测细胞增殖和集落形成能力,划痕实验和Transwell侵袭实验检测细胞的迁移和侵袭能力。结果:膀胱癌组织miR-372-3p m RNA的表达显著低于癌旁组织(0.65±0.56 vs 1.76±0.34,P<0.01)。和转染miR-NC膀胱癌细胞相比,转染miR-372-3p mimics膀胱癌细胞的miR-372-3p m RNA表达显著增加,ATAD2 m RNA和蛋白的表达显著降低,E-cadherin蛋白表达上调,N-cadherin蛋白表达下调,细胞周期明显阻滞,细胞集落形成和增殖能力显著降低,细胞迁移数和侵袭数减少。结论:miR-372-3p的低表达可能与膀胱癌的发生发展有关,其通过靶向调控ATAD2抑制膀胱癌细胞的增殖、迁移和侵袭能力,可能成为膀胱癌生物治疗的新靶标。Objective： To detect expression of miR-372-3p in the bladder cancer cell transfected with miR-372-3p mimics and to ex- plore effect of miR-372-3p on proliferation, migration and invasion of the bladder cancer 5637 and T24 cell lines. Methods： Using lipo- fection, miR-372-3p mimics or miR-NC was transfected into the bladder cancer 5637 and T24 cells. Expressions of miR-372-3p mRNA and ATAD2 mRNA, expressions ofADAD2, E-cadherin and N-cadherin proteins, cell cycle distribution, viability of cell, cell prolifera- tion ability, cell migration ability and cell invasion ability in the bladder cancer cells transfected with miR-372-3p mimics and miR-NC were respectively detected by qPCR, Western blotting assay, flow cytometry, MTT assay, colony forming test, scratch assay and Tran- swell assay. Results： Comparing with the bladder cancer cell transfected with miR-NC, expression of miR-372-3p mRNA significantly increased, expressions ofATAD2 mRNA and protein obviously decreased, expression of E-cadherin protein raised, expression of N-cad- herin protein came down, cell cycle obviously arrested, abilities of cell viability and proliferation markedly reduced as well as cell num- bers of migration and invasion reduced, in the bladder cancer cell transfected with miR-372-3p mimics. Conclusion： Low expression of miR-372-3p might be associated with occurrence and development of the bladder carcinoma, miR-372-3p could inhibit abilities of pro- liferation, migration and invasion of the bladder cancer cell through targeting control. It could be a novel target in biotherapy of the bladder carcinoma.

关 键 词：miR-372-3p 膀胱癌 5637细胞 T24细胞 增殖 迁移 侵袭 

分 类 号：R737.14[医药卫生—肿瘤] R730.2[医药卫生—临床医学]