HPLC测定混合型饲料添加剂中L-苹果酸含量  

Determination of L-malic acid content in mixed feed additive by HPLC

作　　者：杨建平 陈鲜鑫 刘星 

机构地区：[1]四川美嘉龙生物科技有限公司,四川乐山614000 [2]乐山市农业科学研究院,四川乐山614000 [3]四川省乐山市农业局,四川乐山614000

出　　处：《饲料工业》2018年第2期61-64,共4页Feed Industry

摘　　要：用HPLC法测定混合型饲料添加剂中L-苹果酸(malic acid,LMA)的含量。采用C18(250 mm×4.6 mm×5μm)色谱柱,以0.02 mol/l磷酸二氢钾:乙腈=982(v:v),流速0.8 ml/min,柱温25℃,检测波长210 nm,进样量10μl。结果表明:L-苹果酸对照品和样品的保留时间均是3.9 min,其线性方程分别为Y=0.390 9X-4.578 6,R=0.999 8(n=5),在126.25~2 020μg/ml的范围内,线性关系良好,其最低检测量为12.23μg/ml。精密度的RSD为0.43%,表明此方法精密度较好;重现性的RSD为0.17%,表明重现性良好;回收率的RSD为0.61%。用此方法检测出其混合饲料添加剂中L-苹果酸含量为(99.01±0.11)%,符合产品规定的质量要求。该实验建立的HPLC方法可快速、准确测定混合饲料中L-苹果酸含量,为其质量控制提供科学依据。The test was to determine the content of malic acid （LMA） in mixed feed additive by HPLC. The determined conditions were following： C18 column （250 mm × 4.6 mm× 5 μm）; mobile phrase： 0.02 mol/1 potassium dihydrogen phosphate ： aeetonitrile=98 ： 2 （v ： v）; flow rate： 0.8 ml/min, column temperature： 25 ℃, detection wavelength： 210 nm, the injection volume： 10 μl. The retention times of Lmalic acid reference substance and sample were 3.9 min, and the linear equations were Y=0.390 9X- 4.578 6, R=0.999 8 （n=5）, the linear relationship is good in the range of 126.25-2 020 μg/ml, the minimum detection was 12.23 μg/ml. The RSD of the precision was 0.43%, which indicated that the precision of this method was good. The RSD of reproducibility was 0.17%, which indicated that the reproducibility was good. The RSD of the recovery was 0.61%. The content of L-malic acid in the mixed feed additive was （99.01±0.11）%, which was in accordance with the quality requirement of the product. The HPLC method established in this experiment can be used to determine the rapid and accurate determination of L-malic acid in mixed feed and provide scientific basis for its quality control.

关 键 词：L-苹果酸 高效液相色谱 混合饲料 添加剂 

分 类 号：S816.17[农业科学—饲料科学]