基于iTRAQ技术研究滋阴降火方药知柏地黄丸对阴虚“上火”SD大鼠血清蛋白组的影响  

Study on Ziyin Jianghuo Formula Zhibai Dihuang Pill on Serum Protein of SD Rats with Yin-deficiency Shanghuo Based on iTRAQ

作　　者：刘昌铭 毛连根 杨粟 江婷婷 陈钟梁[1] 屠慧惠 陈静 胡玉婷 甘霖 李仲杰[1] 李继承[1,2] 

机构地区：[1]浙江大学细胞生物学研究所,杭州310058 [2]华南理工大学医学院,广州510006

出　　处：《世界中医药》2017年第12期2876-2880,共5页World Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2014CB543002)

摘　　要：目的:研究知柏地黄丸治疗阴虚"上火"的生物学机制。方法:应用iTRAQ-2DLC-MS/MS蛋白组学技术检测阴虚"上火"大鼠在知柏地黄丸治疗前后的血清蛋白组学表达谱,筛选并鉴定与知柏地黄丸治疗作用相关的蛋白质群,并应用生物信息学对差异表达的蛋白进行功能富集分析,揭示知柏地黄丸对阴虚"上火"治疗作用的生物学机制。结果:与正常大鼠比较,阴虚"上火"对照组大鼠血清中有47个差异蛋白,其中有20个表达上调,27个表达下调。知柏地黄丸治疗后,有19个差异蛋白恢复至正常或接近正常水平。通过生物信息学分析,发现这些蛋白主要集中在免疫反应与物质代谢过程。其中参与免疫反应的蛋白主要有有补体成分4(C4)、凝血因子X(F10)、激酶相关磷蛋白2(Skap2);参与代谢作用的蛋白有L乳酸脱氢酶A链(Ldha)、果糖二磷酸醛缩酶A(Aldoa)、花生四烯酸脂肪氧合酶(Alox12)、GDP分解酶抑制剂1(Arhgdia)、肌球蛋白轻链(Myl6)。结论:阴虚"上火"主要表现为机体免疫反应与物质代谢发生紊乱,知柏地黄丸可以通过调节免疫与物质代谢反应治疗阴虚"上火"。Objective： To explore the therapeutic mechanism of Zhibai Dihuang Granule（ ZDG） in Yin-deficiency-heat（ YDH）syndrome. Methods： ITRAQ-2 DLC-MS/MS technique was applied to screen the differentially expressed serum proteins between ZDG treated rats and YDH syndrome rats. The serum protein profiles were analyzed by bioinformatics tools,such as Gene Ontology（ GO） database,Kyoto Encyclopedia of Genes and Genomes（ KEGG） pathway database. Compared with normal rats,47 differentially expressed proteins were shown in the serum of YDH syndrome rats,including 20 up-regulated proteins and 27 down-regulated proteins. Nineteen proteins returned to normal after ZDG treatment,which mostly involved in immune reaction and metabolism.Complement C4（ C4）,coagulation factor X（ F10）,and Src kinase-associated phosphoprotein 2（ Skap2） participated in immune response,while L-lactate dehydrogenase A chain（ Ldha）,Fructose-bisphosphate aldolase A（ Aldoa）,Arachidonate 12-lipoxygenase（ Alox12）,Rho GDP-dissociation inhibitor 1（ Arhgdia）,and Myosin light polypeptide 6（ Myl6） mainly related to metabolic process. Conclusion： YDH syndrome is highly associated with the disturbance of immune function and metabolism,and ZDG treats YDH syndrome mostly through regulating immune activity and metabolic process.

关 键 词：阴虚“上火” 知柏地黄丸 蛋白组学 ITRAQ 免疫 代谢 

分 类 号：R22[医药卫生—中医基础理论]