基于iTRAQ技术的中医实热“上火”血清蛋白质组学特征分析  

Analysis of Serum Proteomics Features of “Shanghuo” in Traditional Chinese Medicine Based on iTRAQ Technology

作　　者：陈娟[1] 徐莉[1] 谢冠群[1] 周佳[1] 谢志军[1] 甘静[1] 范永升[1] 

机构地区：[1]浙江中医药大学基础医学院,杭州310053

出　　处：《世界中医药》2017年第12期2897-2901,2907,共6页World Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2014CB543001);973计划子课题(2014CB543001-1);浙江中医药大学基础医学院科技创新团队项目(JCIT2016-1)

摘　　要：目的:筛选实热"上火"血清差异表达蛋白,为实热"上火"机制的深入研究提供科学依据。方法:采用相对和绝对定量同位素标记(iTRAQ)对血清蛋白质进行检测,结合生物信息学分析(GO、STRING)筛选实热"上火"与正常非"上火"人群差异显著的蛋白质。结果:与正常对照组比较,实热上火组血清筛选出49个差异蛋白质,其中上调的22个,下调的27个。载脂蛋白C3(Apo C3)、乳酸脱氢酶(LDH)和维生素K依赖蛋白S(PROS1)等显著上调,而载脂蛋白A4(Apo A4)、超氧化物歧化酶3(SOD3)和纤溶酶原激活物抑制剂1(PAI-1)等显著下调。结论:观察组脂质代谢、糖酵解异常与氧化应激、炎性反应的发生密切相关,且凝血功能下降,易造成出血,筛选的差异蛋白可为实热"上火"的生物学基础研究提供依据。Objective： To screen differentially expressed proteins in excessive heat＂shanghuo＂serum and provide a scientific basis for in-depth study on the mechanism of excessive heat＂shanghuo＂. Methods： Serum proteins were detected by relative and absolute quantitative isotope labeling（ iTRAQ）,and bioinformatics analysis（ GO,STRING） was used to screen the proteins that were significantly different between the normal fever group and normal non-lit group subjects. Results： Compared with the normal control group,49 differentially expressed proteins were screened by excessive heat ＂Shanghuo＂group,with 22 up-regulated and27 down-regulated. Apolipoprotein C3（ Apo C3）,lactate dehydrogenase（ LDH）,and vitamin K-dependent protein S（ PROS1）were significantly up-regulated while Apo A4,SOD3 and the plasminogen activator inhibitor 1（ PAI-1） and so on were significantly down-regulated. Conclusion： The abnormal lipid metabolism and glycolytic abnormality in the＂shanghuo＂crowd are closely related to the occurrence of oxidative stress and inflammation,and the coagulation function is decreased,leading to easily bleeding.The differential proteins screened can provide references for biological foundation of basic research on ＂shanghuo＂.

关 键 词：实热上火 ITRAQ 血清蛋白质组学 生物标志物 

分 类 号：R22[医药卫生—中医基础理论] R24[医药卫生—中医学]