黄檗幼苗抗氧化物质及生物碱含量对UV-B辐射增强响应  

Influence on antioxidants and alkaloid content of Phellodendron amurense seedlings grown under supplementary UV-B radiation

作　　者：张玉红 程楷珊 高欣 张锡国 刘彤[2] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040 [2]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《北京林业大学学报》2018年第1期27-36,共10页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(31370630);林业公益性行业科研专项(201104066)

摘　　要：【目的】本文以东北重要药用植物黄檗幼苗为对象,研究黄檗幼苗中抗氧化物质以及主要药用活性成分含量变化对增补不同的UV-B辐射强度的响应。【方法】通过在温室内控制实验,设置了3个UV-B辐射强度梯度的处理,分别是对照组(CK,自然光照)、低辐射组(T1,UV-B辐射强度为3.26μW/cm2)和高辐射组(T2,UV-B辐射强度为9.78μW/cm^2),研究黄檗幼苗中的抗氧化物质及主要有效活性成分,即3种生物碱(小檗碱、药根碱和掌叶防已碱)含量对不同UV-B辐射强度的响应。【结果】增补UV-B辐射会使黄檗幼苗中的羟基自由基、丙二醛、单宁、可溶性蛋白含量明显增加并随辐射强度升高而增加;除第40天处理外,不同处理间黄檗幼苗根中总黄酮含量随紫外辐射强度呈下降趋势,而茎中总黄酮含量随辐射强度增强而升高,叶中总黄酮含量随辐射强度升高和处理时间延长而升高;在辐射处理40 d时,黄檗幼苗茎中3种生物碱含量都高于对照,低强度辐射的T1组含量高于高强度辐射的T2组且差异显著(P<0.05)。根和叶中3种生物碱在辐射处理40 d时虽随辐射时间增长含量有所升高但仍低于对照且差异显著(P<0.05)。【结论】UV-B辐射增强会导致药用植物黄檗不同器官中的抗氧化物质和药用活性成分含量发生不同的变化,这对以后黄檗不同利用模式的大规模种植有着重要意义,同时也对今后的药用植物中生物碱的利用有着深远影响。[ Objective] The rapid development of the global industry has led to the enhancement of UV- B radiation in the atmosphere, which directly affects the growth and development of plants and their physiological activities. All kinds of secondary metabolites in plants are greatly influenced by the environment, how to respond to the enhancement of UV-B radiation is also increasingly emphasized by the planting industry. In this paper, Phellodendron amurense, the important medicinal plant in the Northeast China was taken as the object of research to study the responses of the contents of antioxidant substances and main medicinal active components in the seedlings of P. amurense to different intensities of UV-B radiation. [Method] Experiments were carried out in greenhouse with controlled environment. Three levels of UV-B radiation intensity treatments were set as control （CK, natural light）, low radiation （T1, supplement UV-B radiation intensity 3.26 I^W/cm2 , and high radiation（T2, supplement UV-B radiation intensity 9.78 p^W/cm2, to study the responses of antioxidants and the main active ingredients （ three alkaloids of bererine, jatrorrhizine and palmatine） contents in P. amurense seedlings to different UV-B radiation intensity. [ Result] The results showed that the content of malondialdehyde （MDA）, hydroxyl radical, tannin and soluble protein in P. amurense seedlings significantly increased along with the radiation intensity. The total flavonoid contents in the root of P. amurense seedlings declined with UV-B radiation intensity, except those in treatments of 40 days. On the contrary, the total flavonoid contents in the stem rose with radiation intensity. At the time of radiation treatment for 40 days, the contents of three alkaloids in the stem of P. amurense seedlings were higher than in the CK group, and the T1 group with low intensity radiation was higher than the T2 group with high intensity radiation （P 〈 0.05 ）. The content of three alkaloids in the root and leaf increased with the 40 day

关 键 词：黄檗幼苗 UV-B辐射 抗氧化成分 小檗碱 药根碱 掌叶防已碱 

分 类 号：S718.43[农业科学—林学] S792.31