南宁市汉族女性不明原因复发性流产与MTHFR基因C677T位点多态性相关性研究  

Association between MTHFR C677T polymorphism and unexplained recurrent spontaneous abortion in Nanning Han patients

作　　者：蒋武[1] 鲁衍强 李瑛 朱茂灵 覃頔[1] 黄永全[1] 沈寅琛[1] 黄卫彤[1] 杨琦[3] 

机构地区：[1]广西南宁市妇幼保健院,南宁530011 [2]上海靶向分子医学研究,上海200433 [3]中国疾病预防控制中心妇幼保健中心,北京100101

出　　处：《广西医科大学学报》2017年第12期1728-1730,共3页Journal of Guangxi Medical University

基　　金：广西科学研究与技术开发计划项目(No.14124004-1-16);中国疾病预防控制中心妇幼保健中心妇幼保健分子遗传医学研究专项计划(No.FY-ZX-ZD-0102)

摘　　要：目的:探讨南宁市汉族女性不明原因复发性流产与亚甲基四氢叶酸还原酶(MTHFR)基因C677T位点多态性的关联性。方法:采用病例对照研究的方法,以南宁市汉族女性152例不明原因复发性流产患者(流产组)和313例正常妇女(对照组)为研究对象,用荧光定量PCR技术,检测MTHFR基因C677T位点的单核苷酸多态性,并分析检测结果与不明原因复发性流产的相关性。结果:流产组MTHFR 677TT基因型占比(10.5%)较对照组(6.4%)明显升高,同时T等位基因在流产组的占比(30.3%)也显著高于对照组(21.7%),该位点基因型(CC、CT、TT)、等位基因(C、T)在两组中的分布差异均有统计学意义(P<0.05)。CT基因型者发生流产的风险为CC基因型的1.620倍,TT基因型者发生流产的风险为CC基因型的2.074倍。结论:南宁市汉族女性不明原因复发性流产的发生与MTHFR C677T位点的多态性变化具有相关性。Objective： To investigate the correlation between the 5, 10-methylenetetrahydrofolate reductase （MTHFR） C677T polymorphism and unexplained recurrent spontaneous abortion （URSA） in Han individuals in Nanning City. Methods： 152 patients with URSA and 313 normal women were enrolled. Gene polymorphisms of MTHFR was analyzed by fluorescence quantitative PCR. Results： A statistically significant increase in the frequency of the MTHFR 677TT genotype was found in URSA patients （10. 5%）, compared to controls （6. 4%）, and the T allele frequency was notably higher as well. There was significant difference in the distribution of genotypes and alleles of MTHFR C677T between URSA patients and controls （P〈0. 05）. The women with CT genotype had 1. 620 times higher risk for developing URSA than those with CC genotype, and TT genotype was 2. 074 times higher. Conclusion： The polymorphisms of MTHFR C677T was associated with URSA in Nanning Han individuals.

关 键 词：南宁市汉族女性 不明原因复发性流产 亚甲基四氢叶酸还原酶 单核苷酸多态性 

分 类 号：R714.21[医药卫生—妇产科学]