常用可摘局部义齿支架材料表面微生物粘附的实验研究  

Adhesion of microorganisms to the surface of removable partial denture metal materials in vitro

作　　者：张雪[1] 孙宇[1] 张兆钰 崔丹[1] 喻望清 焦二龙 肖睿 

机构地区：[1]哈尔滨医科大学口腔医院修复科,黑龙江哈尔滨150001

出　　处：《口腔医学》2018年第1期19-22,共4页Stomatology

基　　金：黑龙江省教育厅科学技术研究项目(12531322)

摘　　要：目的研究变异链球菌和白色念珠菌对3种口腔常用金属材料的粘附性,为临床选择合适的材料提供理论依据。方法选取最常用做可摘局部义齿支架材料的3种金属——钴铬合金、Vitallium 2000材料、纯钛,分别制成尺寸为20 mm×10mm×2 mm的试件,每组各6个。将各组试件抛光,并保证各组间粗糙度无明显差异。选取变异链球菌和白色念珠菌作为实验菌株,制成菌悬液,分别放入试件进行48 h的粘附实验,取出试件后冲洗、震荡、培养,采用菌落形成计数法(CFU)测定微生物表面粘附量。结果粘附48 h后,试件表面变异链球菌和白色念珠菌的粘附数量均为钴铬合金>纯钛>Vitallium 2000材料(P<0.05)。结论 Vitallium 2000材料与钴铬合金和纯钛相比,粘附微生物的数量最少,值得应用推广。Objective To compare the adhesion ability of microorganisms to three different metal materials. Streptococcus mutans and Candida albicans were used in adhesion experiment in order to provide theoretical guidance for choosing the suitable clinical materials. Methods Three types of removable partial denture metal materials were selected, including Co-Cr alloy, Vitallium 2000 and pure tita- nium. 20 minx 10 mmx2.0 mm specimens of these dental materials were prepared, six test pieces in each material. Polishing was made to ensure that there was no obvious difference. Streptococcus mutaus and Candida albicans, as experimental strains, were placed on sus- pension over plate and taken in culture for 48 hours, the amount of bacteria adhering to the specimen surface was assayed by means of clone forming unit （CFU） method after being eluted, shaked, and cultured. Results After 48 hours, the sequence of the adhesive ca- pacity of the oral microorganisms on the surface of specimens was：Co-Cr alloy〉pure titanium〉Vitallium 2000（P〈0.05）. Conclusion Compared with Co-Cr alloy and pure titanium, Vitallium 2000 can decrease the adhesion of microorganisms, and it is worth promoting and applying.

关 键 词：微生物粘附 VITALLIUM 2000 活动义齿 材料 

分 类 号：R783.1[医药卫生—口腔医学]