RAGE基因多态性与2型糖尿病伴慢性牙周炎遗传易感性的相关研究  

A study on the association between RAGE gene polymorphisms and type 2 diabetes mellitus with chronic periodontitis

作　　者：俞宁[1] 孙尚敏[1] 刘博[1] 钟慧敏[1] 潘亚萍[1] 钟鸣[2] 

机构地区：[1]中国医科大学口腔医学院牙周科辽宁省口腔医学研究所牙周病研究室,辽宁沈阳110002 [2]中国医科大学口腔医学院中心实验室,辽宁沈阳110002

出　　处：《口腔医学》2018年第1期33-38,共6页Stomatology

基　　金：辽宁省牙周病诊疗能力提高项目(LNCCC-C08-2015)

摘　　要：目的探讨晚期糖基化终产物受体(receptor for advanced glycation end products,RAGE)基因的5个SNP位点在2型糖尿病伴慢性牙周炎、单纯慢性牙周炎、健康对照北方汉族人群中分布的差异,从而研究RAGE基因是否为糖尿病和牙周炎可能的致病易感基因。方法运用飞行时间质谱法检测19例2型糖尿病伴慢性牙周炎患者(DM组),22例单纯慢性牙周炎组(CP组)以及54例健康对照组(H组)全血基因组DNA中5个单核苷酸多态性(single nucleotide polymorphism,SNP)位点。结果rs17846808、rs1800625、rs184003、rs2070600、rs3134940基因多态性在三组间分布无差异。DM组中的rs3134940 A/A(野生纯合子)基因型人群的龈沟出血指数(sulcus bleeding index,SBI)显著高于(A/G+G/G,杂合子和突变纯合子)基因型人群的SBI。CP组中的rs1800625(T/C+C/C)和rs3134940(A/G+G/G,杂合子和突变纯合子)基因型人群缺失牙数显著性增高。结论RAGE基因并非2型糖尿病以及慢性牙周炎的易感基因。rs3134940 G等位基因可能在2型糖尿病伴慢性牙周炎进展过程中起到一定保护作用。Objective To investigate the differential distributions of five single nucleotide polymorphism （SNP） loci of RAGE gene in type 2 diabetes with chronic periodontitis （DM）, chronic periodontitis （CP）, and healthy controls among northern Chinese Han peo- ple, so as to study whether RAGE gene serves as a susceptible candidate gene for both diabetes and periodontitis. Methods A flight- time mass spectrometer method was utilized to analyze five SNPs of 19 DM subjects, 22 CP subjects, and 54 healthy subjects. Results The distributions of rs17846808, rs1800625, rs184003, rs2070600, rs3134940 polymorphisms demonstrated no significance among three groups. In DM group, subjects carrying rs3134940 A/A genotype had higher （ sulcus bleeding index, SBI） than those carrying A./G＋G/G genotypes. In CP group, subjects carrying rs1800625 （T/C＋C/C） and rs3134940 （A./G＋G/G） genotypes had significantly higher loss of teeth. Conclusion In the samples of this study, RAGE gene fails to demonstrate its potential to be a candidate gene for type 2 diabetes and chronic periodontitis susceptibilities, rs3134940 G allele may have some protective roles in the progression of type 2 diabetes with chronic periodontitis.

关 键 词：基因多态性 2型糖尿病 慢性牙周炎 晚期糖基化终产物受体 

分 类 号：R780.2[医药卫生—口腔医学]