KISS-1基因对人肺鳞状细胞癌细胞YTMLC-90增殖及侵袭能力的影响  

Effect of KISS-1 gene on proliferation and invasion of human lung squamous carcinoma cell line YTMLC-90

作　　者：赵彬 王俊然 郑素琴 

机构地区：[1]华北理工大学校医院,河北省唐山市063000 [2]华北理工大学基础医学院病理学系,河北省唐山市063000 [3]河北省唐山市妇幼保健院病理科,063000

出　　处：《中国医药》2018年第1期48-50,共3页China Medicine

基　　金：河北省自然科学基金(H2014209247)~~

摘　　要：目的探讨肿瘤转移抑制基因KISS-1对人肺鳞状细胞癌细胞YTMLC-90增殖及侵袭能力的影响。方法通过基因转染技术,将携带KISS-1基因的pEGFP-N2-KISS-1质粒转染入人YTMLC-90细胞,同时设不携带KISS-1基因的空质粒转染组和空白对照组作为对照,用蛋白质印迹法检测转染后YTMLC-90细胞中KISS-1蛋白的表达以确定转染是否成功;噻唑蓝(MTT)法测定3组YTMLC-90细胞增殖情况;Millicell小室法检测3组YTMLC-90细胞的侵袭能力。结果成功将KISS-1基因转染入YTMLC-90细胞。pEGFP-N2-KISS-1质粒转染组YTMLC-90细胞的增殖活性与空质粒转染组和空白对照组差异无统计学意义(P>0.05),但YTMLC-90细胞穿透Millicell聚碳酸酯膜的细胞数较空质粒转染组和空白对照组明显减少[(35±5)×10~5个比(82±6)×10~5个、(84±5)×10~5个],差异有统计学意义(P<0.05)。结论 KISS-1基因对YTMLC-90细胞的增殖能力没有影响,但对其侵袭能力有抑制作用,提示KISS-1基因能够抑制肺癌的侵袭。Objective To investigate the effect of KISS-1 gene on proliferation and invasion of human lung squamous carcinoma cell line YTMLC-90. Methods The pEGFP-N2-KISS-1 plasmid carried with KISS-1 gene and empty plasmid were respectively transfected into human YTMLC-90 cells; YTMLC-90 cells without special treatment were as a blank control group. Expression of KISS-1 protein was detected by western blot method to determine whether the transfection was successful. Proliferation activity of YTMLC-90 cells was tested by the MTF assay; invasion ability was tested by Millicell chamber method. Results KISS-1 gene was successfully transfected into YTMLC-90 cells it/pEGFP-N2-KISS-1 plasmid group. The proliferation activity in pEGFP-N2-KISS-1 plasmid group had no significant differences compared to that in empty plasmid group and blank control group（ P 〉 0. 05 ）. The number of ceils that passed through polycarbonate membrane of Millicell chamber in pEGFP-N2-KISS-1 plasmid group was significantly less than that in empty plasmid group and blank control group[ （35 ±5） ×10^5 vs （ 82 ± 6）×10^5, （ 84 ± 5 ） ×10^5 ] （ P 〈 0. 05 ）. Conclusion KISS-1 gene has no effect on proliferation but inhibits invasion of YTMLC-90 cells, suggesting it may inhibit the invasion of lung cancer.

关 键 词：肺肿瘤 YTMLC-90细胞 KISS-1基因 基因转染 

分 类 号：R734.2[医药卫生—肿瘤]