硼酸钠对人肝癌细胞HepG2内质网应激蛋白Grp78和CHOP表达的影响  

Effects of borax on expression of endoplasmic reticulum stress proteins Grp78 and CHOP in hepatoma carcinoma cells HepG2

作　　者：王生 武伦 魏英 魏广民 张有顺 袁方均 WANG Sheng;WU Lun;WEI Ying;WEI Guangmin;ZHANG Youshun;YUAN Fangjun(DongFeng Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, Chin)

机构地区：[1]湖北医药学院附属东风医院,湖北十堰442000

出　　处：《山东医药》2018年第2期9-12,共4页Shandong Medical Journal

基　　金：湖北省中青年人才项目资助(Q20162112);湖北省十堰市科技局引导性科研性项目(17Y50)

摘　　要：目的观察硼酸钠(Borax)对人肝癌细胞HepG2内质网应激(ERS)蛋白葡萄糖调节蛋白78(Grp78)及下游靶基因C/EBP家族同源蛋白(CHOP)表达的影响,探讨其促凋亡的机制。方法将HepG2细胞分为两组,观察组用0.1 mmol/L Borax处理,对照组未经Borax处理,分别采用qRT-PCR法和免疫印迹法检测观察组Borax作用1、2、3、4 h和对照组HepG2细胞中Grp78、CHOP mRNA和蛋白表达。结果与对照组比较,观察组Grp78 mRNA相对表达量在Borax作用2 h达到最高,在作用3、4 h下降(F=29.233,P<0.01);与对照组比较,观察组CHOP mRNA相对表达量在Borax作用4 h内表达持续升高(F=208.207,P<0.01)。与对照组比较,观察组Grp78蛋白相对表达量在Borax作用2 h内表达上调,在作用3、4 h下降(F=79.936,P<0.01);与对照组比较,CHOP蛋白相对表达量在Borax作用4 h内持续升高(F=200.324,P<0.01)。结论 Borax通过活化ERS蛋白Grp78,并进一步激活促凋亡蛋白CHOP,从而启动ERS依赖的凋亡途径,诱导HepG2细胞凋亡。Objective To observe the effects of borax on the expression of endoplasmic reticulum stress（ERS） proteins glucose-related protein 78（Grp78） and C/EBP homologous protein（CHOP） in human hepatocellular carcinoma cell line HepG2,and to explore the mechanism of borax-induced apoptosis.Methods HepG2 cells were divided into the observation group（treated with 0.1 mmol/L borax） and control group（without treatment of borax）.The mRNA and protein levels of Grp78 and CHOP in both of the two groups were detected by real-time quantitative PCR（qRT-PCR） and Western blotting at 1,2,3 and 4 h after treatment.Results Compared with the control group,the mRNA expression of Grp78 reached the peak at 2 h,and decreased at 3 and 4 h（F = 29.233,P〈 0.01）; the mRNA expression of CHOP gradually increased in the observation group within 4-hour treatment（F = 208.207,P〈 0.01）.Compared with the control group,the protein expression of Grp78 reached the peak at 2 h,and decreased at 3 and 4 h（F = 79.936,P〈 0.01）; the protein expression of CHOP gradually increased in the observation group within 4-hour treatment（F = 200.324,P〈 0.01）.Conclusion Borax starts the ERS-dependent apoptotic pathway and induces the apoptosis of HepG2 cells by activating the ERS protein Grp78 and further activating the pro-apoptotic protein CHOP.

关 键 词：硼酸钠 内质网应激 HEPG2细胞 细胞凋亡 葡萄糖调节蛋白78 C/EBP家族同源蛋白 

分 类 号：R735.7[医药卫生—肿瘤]