贵州3个世居少数民族CCR5基因Δ32多态分布  

Genotyping and Polymorphism of HIV-1 related CCR5Δ32 allele in Three Ethnic Groups in Guizhou

作　　者：国学红 何燕[1] 张秀秀 王婵娟 张婷[1] 官志忠[1] 

机构地区：[1]地方病与少数民族性疾病教育部重点实验室(贵州医科大学) [2]贵州省医学分子生物学重点实验室,贵州贵阳550004

出　　处：《广东医学》2018年第1期88-91,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:31560306);贵阳市科技计划项目(编号:筑科合同[2017]5-9)

摘　　要：目的调查贵州3个世居少数民族人群(苗族、布依族、水族)中趋化因子受体5(CCR5)基因Δ32位点多态性分布情况。方法采用PCR法直接扩增120例男性样本(雷山苗族27例、荔波布依族70例、荔波水族23例)的CCR5Δ32等位基因,分析PCR扩增产物并对产物进行测序验证。结果 3个世居少数民族人群中只发现了169 bp片段的野生型基因型,CCR5Δ32等位基因突变频率为0。结论贵州3个世居少数民族人群CCR5Δ32等位基因突变频率较低,可能对人类免疫缺陷性病毒(HIV-1)易感。Objective To investigate the allelic polymorphism of CCR5Δ32 in three ethnic groups in Guizhou.Methods The study population contained 120 samples,of which consisted of 27 Miao people,70 BuYi people and 23 Shui people. Polymerase chain reaction was applied for amplifying target gene fragment,as DNA sequencing was used to verify the accuracy of PCR products. Results The frequency of the mutant CCR5Δ32 allele was 0. Neither the heterozygous CCR5-w t/Δ32 mutant nor homozygous were identified in the three ethnic groups. Conclusion The frequency of the mutant CCR5Δ32 allele is very low in the three ethnic groups in Guizhou.

关 键 词：CCR5△32基因 贵州 少数民族 HIV-1 易感性 

分 类 号：R394[医药卫生—医学遗传学]